Immunoassay

Authors

Puji Astuti
Departemen Biokimia, Fakultas Kedokteran Universitas Tanjungpura
https://orcid.org/0000-0002-2444-2255
Sari Eka Pratiwi
Universitas Tanjungpura
https://orcid.org/0000-0003-4577-8863

Keywords:

Imunologi , Imunoglobulin, Immunoassay

Synopsis

Dalam era modern ini, ilmu pengetahuan dan teknologi terus berkembang dengan pesat, membawa perubahan signifikan dalam berbagai bidang, termasuk kedokteran dan biologi molekular. Salah satu perkembangan yang menonjol adalah teknik immunoassay, yang telah menjadi alat penting dalam penelitian dan diagnosis medis. Buku ini hadir untuk memberikan wawasan mendalam tentang dasar-dasar imunologi dan aplikasi praktis dari teknik immunoassay.

Buku ini dimulai dengan pengantar imunologi termasuk pembahasan terkait limfosit B dan produksi antibodi yang dirancang untuk memberikan landasan yang kuat bagi pembaca dalam memahami konsep-konsep dasar yang akan digunakan dalam bab-bab selanjutnya terutama terkait bagaimana immunoassay bekerja. Bagian selanjutnya pada buku ini membahas prinsip interaksi antigen-antibodi, serta berbagai teknik pemeriksaan imunologi pada sampel jaringan, sel, dan cairan tubuh.

Kami berharap buku ini dapat menjadi referensi yang bermanfaat bagi mahasiswa, peneliti, dan praktisi di bidang kedokteran dan biologi molekular. Dengan pemahaman yang baik tentang prinsip dan aplikasi teknik immunoassay, diharapkan pembaca dapat mengembangkan dan mengaplikasikan pengetahuan ini untuk kemajuan ilmu pengetahuan dan peningkatan kesehatan masyarakat.

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Author Biographies

Puji Astuti, Departemen Biokimia, Fakultas Kedokteran Universitas Tanjungpura

Puji Astuti, menempuh pendidikan sarjana di program studi Biokimia IPB University dan pendidikan magister di bidang Biomedical Science di Maastricht University, Belanda. Penulis aktif mengajar
sebagai dosen sejak tahun 2019 di Program Studi D-IV Teknologi laboratorium Medik, Politeknik ‘Aisyiyah Pontianak hingga 2022. Pada tahun 2022 penulis mulai aktif bekerja sebagai dosen di program studi kedokteran Universitas Tanjungpura dengan bidang keahlian biokimia dan biologi molekuler. Hingga saat ini selain aktif sebagai dosen, penulis juga aktif melakukan berbagai riset baik di bidang biokimia maupun di bidang lainnya.

Sari Eka Pratiwi, Universitas Tanjungpura

Sari Eka Pratiwi, menyelesaikan gelar Sarjana Kedokteran dan Profesi dokter di Fakultas Kedokteran Universitas Tanjungpura Pontianak dan mendapatkan gelar Master Ilmu Biomedik di FK-KMK Universitas Gadjah Mada Yogyakarta. Aktif sebagai dosen di Program Studi Kedokteran Fakultas Kedokteran Universitas Tanjungpura sejak tahun 2012 hingga saat ini. Bidang yang ditekuni adalah kedokteran molekuler yang mencakup biologi sel, genetika dan epigenetika, mikro-RNA, imunologi, imunoonkologi, dan patobiologi serta imuno-informatika di bidang penyakit degeneratif dan kanker. Buku ilmiah yang telah terbit sebelumnya adalah “Kanker dan Perilaku Seksual Berisiko pada Remaja”, “Konsep Dasar Biologi Molekuler Kanker untuk Mahasiswa Kedokteran dan Kesehatan”, “Konsep Dasar Patobiologi Kanker Payudara”, "HPV A-Z" dan “Konsep Dasar Biologi Molekuler Kanker Untuk Mahasiswa Kedokteran dan Kesehatan edisi ke-2". Buku Ajar yang telah terbit sebelumnya berjudul “Membran Sel”, "Organela Sel", dan "Inflamasi, Regenerasi, dan Repair Jaringan".

References

N. Alice Lee, Ivan R. Kennedy. Handbook of Immunoassay Technologies: Approaches, Performances, and Applications. Elsevier Inc; 2018.

Darwish IA. Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances. Int J Biomed Sci IJBS. 2006 Sep;2(3):217–35.

Findlay JW, Smith WC, Lee JW, Nordblom GD, Das I, DeSilva BS, et al. Validation of immunoassays for bioanalysis: a pharmaceutical industry perspective. J Pharm Biomed Anal. 2000 Jan;21(6):1249–73.

Fiets WE, Blankenstein MA, Struikmans H, Ruitenberg HM, Nortier JWR. The prognostic value of hormone receptor detection by enzyme immunoassay and immunohistochemistry: a prospective study in patients with early breast cancer. Int J Biol Markers. 2002 Mar;17(1):24– 32.

Astuti P, Beurskens D, Vajen T, Nicolaes GA, Zhang M, Haenen GR. Protection against neutrophil extracellular trap (NET) toxicity by antioxidant monoHER. Makara J Health Res. 2019;23(2):8.

Gemmink A, Daemen S, Wefers J, Hansen J, van Moorsel D, Astuti P, et al. Twenty-four hour rhythmicity in mitochondrial network connectivity and mitochondrial respiration; a study in human skeletal muscle biopsies of young lean and older individuals with obesity. Mol Metab. 2023 Jun 1;72:101727.

Riondato F, Comazzi S. Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma. Front Vet Sci. 2021;8:600986.

Varadé J, Magadán S, González-Fernández Á. Human immunology and immunotherapy: main achievements and challenges. Cell Mol Immunol. 2021;18(4):805–28.

Hall JE, Hall ME. Guyton and Hall Textbook of Medical Physiology. 14th Editi. Canada: Elsevier; 2021.

Subowo. Sitokin. Imunobiologi. 2014;119(7):157–99.

Subowo. Imunobiologi. 3rd ed. Subowo, editor. Jakarta: Sagung Seto; 2014. 3 p.

Murphy K, Weaver C. Janeway’s Immunobiology. Ninth Edition. 9th editio. New York: Taylor & Francis; 2017. (Garland Science).

Marshall JS, Warrington R, Watson W, Kim HL. An introduction to immunology and immunopathology. Allergy Asthma Clin Immunol. 2018;14.

Rastogi I, Jeon D, Moseman JE, Muralidhar A, Potluri HK, McNeel DG. Role of B cells as antigen presenting cells. Front Immunol. 2022 Sep;13.

Kotsias F, Cebrian I, Alloatti A. Antigen processing and presentation. In: International Review of Cell and Molecular Biology. Elsevier Inc.; 2019. p. 69–121.

Zanna MY, Yasmin AR, Omar AR, Arshad SS, Mariatulqabtiah AR, Nur-Fazila SH, et al. Review of dendritic cells, their role in clinical immunology, and distribution in various animal species. Int J Mol Sci. 2021 Aug;22(15).

Ghosh D, Jiang W, Mukhopadhyay D, Mellins ED. New insights into B cells as antigen presenting cells. Curr Opin Immunol. 2021 Jun;70:129–37.

Guerriero JL. Macrophages. Int Rev Cell Mol Biol. 2019 Jan;342:73–93.

Orakpoghenor O, Avazi DO, Markus T, Olaolu O. Lymphocytes: A Brief Review. Sci J Immunol Immunother. 2019;3(1):005–8.

Pabst R. The bone marrow is not only a primary lymphoid organ: The critical role for T lymphocyte migration and housing of long-term memory plasma cells. Eur J Immunol. 2018 Jul;48(7):1096–100.

Kresno SB. Imunologi: Diagnosis dan Prosedur Laboratorium. Edisi keli. Jakarta: Badan Penerbit FKUI; 2010.

Bery AI, Shepherd HM, Li W, Krupnick AS, Gelman AE, Kreisel D. Role of tertiary lymphoid organs in the regulation of immune responses in the periphery. Cell Mol Life Sci. 2022 Jul;79(7).

Sato Y, Silina K, van den Broek M, Hirahara K, Yanagita M. The roles of tertiary lymphoid structures in chronic diseases. Nat Rev Nephrol. 2023 Aug;19(8): 525–37.

Wicherska pawlowska K, Wrobel T, Rybka J. Toll Like Receptors (TLRs), NOD Like Receptors (NLRs) and RIG I Like Receptors (RLRs) in Innate Immunity. TLRs, NLRs and RLRs Ligands as Immunotherapeutic Agents for Hematopoietic Diseases. Int J Mol Sci. 2021 Dec;22(13397): 1–21.

Sethu S, Govindappa K, Alhaidari M, Pirmohamed M, Park K, Sathish J. Immunogenicity to biologics: Mechanisms, prediction and reduction. Arch Immunol Ther Exp (Warsz). 2012 Oct;60(5):331–44.

Kinker GS, Vitiello GAF, Ferreira WAS, Chaves AS, Cordeiro de Lima VC, Medina T da S. B Cell Orchestration of Anti-tumor Immune Responses: A Matter of Cell Localization and Communication. Front Cell Dev Biol. 2021 Jun;9.

Gaber T, Chen Y, Krauß PL, Buttgereit F. Metabolism of T Lymphocytes in Health and Disease. Int Rev Cell Mol Biol. 2019 Jan;342: 95–148.

Spetz J, Presser AG, Sarosiek KA. T Cells and Regulated Cell Death: Kill or Be Killed. Int Rev Cell Mol Biol. 2019 Jan;342: 27–71.

Aribi M. Introductory Chapter: B-Cells. In: Normal and Malignant B-Cell. IntechOpen; 2020.

James LK. B cells defined by immunoglobulin isotypes. Clin Exp Immunol. 2022;210(3): 230–9.

Tkachenko A, Kupcova K, Havranek O. B Cell Receptor Signaling and Beyond: The Role of Iga (CD79a)/Igb (CD79b) in Normal and Malignant B Cells. Int J Mol Sci. 2024 Jan;25(1).

Owen J, Punt J, Stranford SA, Jones PP. Kuby Immunology. Seventh ed. New York: W.H. Freeman and Company; 2013.

WHO. Implementation of Antigen RDT (Ag-RDT) to detect COVID-19 cases in Indonesia. WHO [Internet]. 2020; Available from:

https://www.who.int/indonesia/news/detail/08-12-2020- implementation-of-antigen-rdt-(ag-rdt)-to-detect-covid-19- cases-in-indonesia

Owen P. Kuby Immunology, 7th Edition [Internet]. Available from: https://books.google.co.id/books?id=-JgatAEACAAJ

Fritz H.Kayser, Kurt A Bienz, Johannes Eckert, Rolf M. Zinkernagel. Medical Microbiology. Stuttgart: Thieme; 2005.

WHO. Implementation of Antigen RDT (Ag-RDT) to detect COVID-19 cases in Indonesia. WHO [Internet]. 2020; Available from:

https://www.who.int/indonesia/news/detail/08-12-2020- implementation-of-antigen-rdt-(ag-rdt)-to-detect-covid-19- cases-in-indonesia

Owen P. Kuby Immunology, 7th Edition [Internet]. Available from: https://books.google.co.id/books?id=-JgatAEACAAJ

Fritz H.Kayser, Kurt A Bienz, Johannes Eckert, Rolf M. Zinkernagel. Medical Microbiology. Stuttgart: Thieme; 2005.

Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019- nCoV and naming it SARS-CoV-2. Nat Microbiol. 2020 Apr;5(4):536–44.

Giovanetti M, Benedetti F, Campisi G, Ciccozzi A, Fabris S, Ceccarelli G, et al. Evolution patterns of SARS-CoV-2: Snapshot on its genom variants. Biochem Biophys Res Commun. 2021 Jan 29;538:88–91.

Papanikolaou V, Chrysovergis A, Ragos V, Tsiambas E, Katsinis S, Manoli A, et al. From delta to Omicron: S1- RBD/S2 mutation/deletion equilibrium in SARS-CoV-2 defined variants. Gene. 2022 Mar 10;814:146134.

Liana DF, Novianry V, Andriani A, Mahyarudin M, Astuti P. Disappearance of Imported Cases of Omicron Lineage BA.2.40 in West Kalimantan, Indonesia. Iran J Med Sci [Internet]. 2023; Available from: https://ijms.sums.ac.ir/article_49634.html

Mesri EA, Feitelson MA, Munger K. Human viral oncogenesis: a cancer hallmarks analysis. Cell Host Microbe. 2014 Mar 12;15(3):266–82.

Gelderblom HR. Structure and Classification of Viruses. In: Baron S, editor. Medical Microbiology. 4th edition. Chapter 41 [Internet]. Galveston (TX): University of Texas Medical Branch at Galveston; 1996. Available from: https://www.ncbi.nlm.nih.gov/books/NBK8174/

Maison DP, Deng Y, Gerschenson M. SARS-CoV-2 and the host-immune response. Front Immunol [Internet]. 2023;14. Available from:

https://www.frontiersin.org/journals/immunology/articles/10

.3389/fimmu.2023.1195871

Qi H, Liu B, Wang X, Zhang L. The humoral response and antibodies against SARS-CoV-2 infection. Nat Immunol. 2022;23(7): 1008–20.

Basile L, Guadalupe-Fernández V, Valdivia Guijarro M, Martinez Mateo A, Ciruela Navas P, Mendioroz Peña J, et al. Diagnostic Performance of Ag-RDTs and NAAT for SARS-CoV2 Identification in Symptomatic Patients in Catalonia. Viruses. 2021 May 14;13(5).

Liu W, Liu L, Kou G, Zheng Y, Ding Y, Ni W, et al. Evaluation of nucleocapsid and spike protein-based enzyme- linked immunosorbent assays for detecting antibodies against SARS-CoV-2. J Clin Microbiol. 2020;58(6):10–1128.

Adil MT, Rahman R, Whitelaw D, Jain V, Al-Taan O, Rashid F, et al. SARS-CoV-2 and the pandemic of COVID-19. Postgrad Med J. 2021 Feb;97(1144): 110–6.

Salton MRJ, Kim KS. Structure. In: Baron S, editor. Medical Microbiology. 4th edition. [Internet]. USA: niversity of Texas Medical Branch at Galveston; 1996. Available from: https://www.ncbi.nlm.nih.gov/books/NBK8477/

Vestby LK, Grønseth T, Simm R, Nesse LL. Bacterial Biofilm and its Role in the Pathogenesis of Disease. Antibiot Basel Switz. 2020 Feb 3;9(2).

Marina Basta, Pavan Annamaraju. Bacterial Spores. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023. Available from: https://www.ncbi.nlm.nih.gov/books/NBK556071/

Murat D, Byrne M, Komeili A. Cell biology of prokaryotic organelles. Cold Spring Harb Perspect Biol. 2010 Oct;2(10):a000422.

Adam S, Astuti P, Amalia P, Sukandiarsyah F. Pengembangan Automated Image Analysis untuk Menentukan Jumlah Bakteri Tahan Asam (BTA) pada Kasus Tubercolosis. J Teknol Inf Dan Ilmu Komput. 2022;9(4):809–18.

Cook GM, Berney M, Gebhard S, Heinemann M, Cox RA, Danilchanka O, et al. Physiology of mycobacteria. Adv Microb Physiol. 2009;55:81–182, 318–9.

Chika Okafor, Ayesan Rewane, Ifeanyi Momodu. Bacillus Calmette Guerin [Internet]. Treasure Island (FL): StatPearls Publishing; 2023. Available from: https://www.ncbi.nlm.nih.gov/books/NBK538185/

Covián C, Fernández-Fierro A, Retamal-Díaz A, Díaz FE, Vasquez AE, Lay MK, et al. BCG-Induced Cross-Protection and Development of Trained Immunity: Implication for Vaccine Design. Front Immunol. 2019;10:2806.

Lourens GB, Ferrell DK. Lymphatic Filariasis. Nurs Clin North Am. 2019 Jun;54(2):181–92.

Ahmad F, Liebau E, Rathaur S. Human immune response against filarial HSP70 and its role in the diagnosis of lymphatic filariasis. Parasite Immunol. 2023 May 1;45(5):e12978.

Reverberi R, Reverberi L. Factors affecting the antigen- antibody reaction. Blood Transfus Trasfus Sangue. 2007 Nov;5(4):227–40.

Sagar Aryal. Antigen-Antibody Interaction- Definition, Stages, Types, Examples. 2022; Available from: https://microbenotes.com/introduction-to-antigen-antibody- reactions/

Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. In: Methods in Molecular Biology. Humana Press Inc.; 2019. p. 289–98.

Kim SW, Roh J, Park CS. Immunohistochemistry for pathologists: Protocols, pitfalls, and tips. J Pathol Transl Med. 2016;50(6):411–8.

Maity B, Sheff D, Fisher RA. Immunostaining. Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments. In: Methods in Cell Biology. Academic Press Inc.; 2013. p. 81–105.

Putri RG, Pratiwi SE, Heriyanto DS, Danarto D, Astuti I, Arfian N, et al. Overexpression of MiR-155-5p and increased number of macrophage population in precancerous prostatic disease. Health Sci J Indones. 2020 Dec;11(2):85–91.

Masuda S, Nakanishi Y. Application of Immunohistochemistry in Clinical Practices as a Standardized Assay for Breast Cancer. Acta Histochem Cytochem. 2023;56(1):1–8.

Betterle C, Zanchetta R. The immunofluorescence techniques in the diagnosis of endocrine autoimmune diseases. Autoimmun Highlights. 2012 Aug;3(2):67–78.

Joshi S, Yu D. Immunofluorescence. In: Basic Science Methods for Clinical Researchers. Elsevier Inc.; 2017. p. 135–50.

Zaqout S, Becker LL, Kaindl AM. Immunofluorescence Staining of Paraffin Sections Step by Step. Front Neuroanat. 2020 Nov;14.

Santegoets SJ, Duurland CL, Jordanova EJ, van Ham VJ, Ehsan I, Loof NM, et al. CD163+ cytokine-producing cDC2 stimulate intratumoral type 1 T cell responses in HPV16- induced oropharyngeal cancer. J Immunother Cancer. 2020 Aug;8(2).

Im K, Mareninov S, Diaz MFP, Yong WH. An introduction to performing immunofluorescence staining. In: Methods in Molecular Biology. Humana Press Inc.; 2019. p. 299–311.

McKinnon KM. Flow Cytometry: An Overview. Curr Protoc Immunol. 2018 Feb 21;120:5.1.1-5.1.11.

AAR Bioquest. Fundamentals of Flow Cytometry. 2019; Available from: https://www.aatbio.com/resources/assaywise/2019-8- 1/fundamentals-of-flow-cytometry

Tabatabaei MS, Ahmed M. Enzyme-Linked Immunosorbent Assay (ELISA). Methods Mol Biol Clifton NJ. 2022;2508:115–34.

DeCaprio J, Kohl TO. Immunoprecipitation. Cold Spring Harb Protoc. 2017 Dec 1;2017(12):pdb.prot098640.

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September 26, 2025
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