Templates
Kultur Jaringan: Penyiapan Bibit Rami Secara Inverto
Keywords:
tanaman obat, agrikultur, pertanian, Kesehatan Holistik, Nanobioteknologi, biologi molekular, penelitianSynopsis
Tanaman serat alam yang dikenal dengan sebutan rami dari spesies Boehmaria nivea sudah banyak dikaji potensinya untuk berbagai bidang, seperti tekstil, pakan, kesehatan, dan berbagai industri kreatif. Proyeksi manfaat tanaman ini di masa depan sangat prospektif untuk dikembangkan pada skala besar. Oleh karena itu, perlu upaya budi daya secara massal.
Buku Kultur Jaringan disiapkan untuk membantu mahasiswa, praktisi lapangan, para pengembang rami, maupun masyarakat umum yang ingin mengenaal lebih lengkap tentang kultur jaringan tanaman serat alam, khususnya rami. Pengenalan kultur jaringan merupakan salah satu langkah sederhana untuk memperkenalkan bioteknologi yang dapat diaplikasikan untuk meningkatkan kesejakteraan manusia dan untuk tujuan konservasi tanaman agroinsustri serat alam.
Downloads
Download data is not yet available.
References
Abbas, B.S. dan S. Ginting. 1981. Influence of Rootstock and Scion on Girth Increment in Rubber Trees. Buletin Balai Penelitian Perkebunan Medan. Vol 12 : 145-152.
Adie, B. A., Pérez-Pérez, J., Pérez-Pérez, M. M., Godoy, M., Sánchez-Serrano, J. J., Schmelz, E. A., & Solano, R. (2007). ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis. The Plant cell, 19(5), 1665–1681
Aguilar, M. L., Espadas, F. L., Coello, J., Maust, B. E., Trejo, C., Robert, M. L., … Me, E. De. (2000). The role of abscisic acid in controlling leaf water loss , survival and growth of micropropagated Tagetes erecta plants when transferred directly to the field. Journal of Experimental Botany, 51(352), 1861–1866
Ahroni, A., A. Zuker, Y. Rozen, H. Shejtman, and A. Vainstein. 1997. An efficient method for adventitious shoot regeneration from stem-segment explants of gypsophila. Plant Cell Tiss. Org. Cult. 49: 101-106.
An, X., Jin, G., Luo, X., Chen, C., Li, W., Zhu, G., … Zhu, G. (2018). Research Progress on Tissue Culture and Genetic Transformation of Ramie Research Progress on Tissue Culture and Genetic Transformation. Journal of Natural Fibers, 00(00), 1–7. https://doi.org/10.1080/15440478.2018.
1534188
Anonim . www.tempointeraktif.com, 2010
Anonim, 2013. Kebijakan Pengembangan Rami Dan Dukungan Pada Pilot Project Pengembangan Rami di Kabupaten Garut Direktorat Budi Daya Tanaman Semusim, Direktorat Jenderal Perkebunan, Departemen Pertanian)
Amaregouda, A., Chetti, M.B. and Salimath, P.M. 1994. Effect of antitranspirants on stomata resistance and frequency, relative water content and pod yield in summer groundnu (Arachis hypogaea L.) Ann. Rev. Pl. Physiol. 8: 18-23.
Balittas, 2015. Komoditas, Serat Batang dan Daun: Balittas. [Online] Available at: http://balittas.litbang.pertanian.go.id/ index.php/id/komoditas/serat-batang-dan-daun
Bhatt I.D., Dhar U. 2000. Combined effect of cytokinin on multiple shoot production from cotyledonary node explants of Bauhinia vahlii. Plant Cell, Tissue Organ Cult. 62: 79-83.
Colijin-Hooymans, C.M., J.C. Hakker, J.J. Tansen, and J.B.M. Cuter. 1994. Competence for regeneration of cucumber cotyledons restricted to specific developmental stages. Plant Cell Tiss. Org. Cult. 39: 211-217.
Deb, C., & Imchen, T. (2010). An Efficient In Vitro Hardening Technique of Tissur Culture Raised Plants. Biotechnology Journal, 9(1), 79–83.
Diettrich, B., Mertinat, H., Luckner, M.: Reduction of water loss during ex vitro acclimatization of micropropagated Digitalis lanata clone plants.-Biochem. Physiol. Pflanz. 188: 23-31, 1992.
Dusi, D.M.A., M.D.E.R.P. Almeida, L. S. Caldas, and E.S. Gander. 1993. Transgenic plants of ramie (Boehmeria nivea
Deng, R., & Donnelly, D. J. (1993). In Vitro Hardening of Red Raspberry by Co Enrichment and Reduced Medium Sucrose Concentration. Biotechnology, 28(1991), 1991–1994.
Fila, G., Ghashghaie, J., Hoarau, J., Cornic, G.: Photosynthesis, leaf conductance and water relations of in vitro cultured grapevine rootstock in relation to acclimatisation.-Physiol. Plant. 102: 411-418, 1998.
Gaud.) obtained by Agrobacterium mediated transformation. Plant Cell Rep. 12: 625-628
Gati, E. (1991). Pengaruh Beberapa Zat Tumbuh Sitokinin (Bap, Kinetin Dan 2 -1p) Pada Perbanyakan Mikro Tanaman Rami. Prosiding Seminar Pengembangan Tanaman Industri, 1(2), 10–11.
Gaud, L. (2014). Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie ( Boehmeria nivea, 1–24. https://doi.org/10.1371/journal.pone.0113768
Gless, C., Lörz H. and A. Jähne-Gärtner. 1998. Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L.). Plant Cell Rep. 17: 441-445.
Goda, K., Sreekala, M. S., Gomes, A., Kaji, T., & Ohgi, J. (2006). Improvement of plant based natural fibers for toughening green composites — Effect of load application during mercerization of ramie fibers. Composite Journal, 37, 2213– 2220. https://doi.org/10.1016/j.compositesa.2005.12.014
George, F.E., M.A. Hall, and Geert-Jan De Klerk. 2008. Plant Propagation by Tissue Culture. 3rd Edition Volume 1. The Background. Springer Publihser. Dordrecht, Netherlands. 501 p.
Gu, X.F. and J.R. Zhang. 2005. An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants. Plant Cell Rep. 23:775-779.
Hao G, Du X, Zhao F et al (2010) Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba. Biotechnol Lett 32:305– 314
Hronkova, M., Zahradnickova, H., Simkova, M. et al. The Role of Abscisic Acid in Acclimation of Plants Cultivated in vitro to ex vitro Conditions. BiologiaPlantarum 46, 535 –541 (2003).
Hazarika, BN 2003, Acclimatization of tissue-cultured plants, Curr. Sci., vol. 85, no. 1.
Huang, J.S. and R.D. Mo. 1981. De novo shoot organogenesis from hypocotyls of ramie (Boehmeria nivea Gaud). Acta Bio. Exp. Sin. 14: 111-114.
Huang, J.S., R.D. Mo, and C.J. Yan. 1980. Research note on invitro plant regeneration from cotyledon and hypocotyls of ramie (Boehmeria nivea Gaud.). Guangxi Agric. Sci. 7: 27
Kemenperin. (2012, August 3). Artikel: Kemenperin. Diambil kembali dari Kementerian Perindustrian: https://kemenperin.go.id/artikel/3983/Indonesia-Kurang-Bahan- Baku-Tekstil
Kim, M.K., H.E. Sommer, B.C. Bongarten, and S.A. Merkle. 1997. High-frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. by thidi- azuron. Plant Cell Rep. 16: 536-540.
Lavanya, M., B.Venkateshwarluand B.P. Devi, 2009. Acclimatization of neem microshoots adaptable to semi-sterile conditions. Indian J. Biotechnol., 8: 218-222.
Li, H., S.J. Murch, and P.K. Saxena. 2000. Thidiazuron-induce de novo shoot organogenesis on seedlings, etiolated hypo cotyls and stem segments of Huang-qin. Plant Cell Tiss. Org. Cult. 62: 169-173.
Litbang Perkebunan (2014, Maret 14). Varietas Unggul Rami; Litbang Perkebunan. Retrieved from Litbang Perkebunan: http://perkebunan.litbang.pertanian.go.id/varietas-unggul- rami/
Marin, J.A., Gella, R., Herrero, M.: Stomatal structure and functioning as a response to environmental changes in acclimatized micropropagated Prunus cerasus L.-Ann. Bot. 62: 663-670, 1988.
Mathur A, Mathur AK, Verma P et al (2008) Biological hardening and genetic fidelity testing of micro-cloned progeny of Chlorophytum borivilianum. Afr J Biotechnol 7:1046–1053
Meryani,Reni. 2005. Inisiasi Dan Penggandaan Tunas Rami (Boehmeria Nivea (L.) Gaud) Pada Berbagai Konsentrasi Sitokinin Melalui Teknik Kultur Jaringan. Jurnal Sigma. 8(3)
Mukherjee, P., Mondal, R., Dutta, S., Meena, K., Roy, M., & Mandal, A. (2018). In vitro Micropropagation in Boehmeria nivea to Generate Safe Planting Materials for Large-scale Cultivation. Plant Breeding, 2018(1), 1–7.
Musaddad, M. A. 2007. Agribisnis Tanaman Rami. Depok: Penebar Swadaya. 82 hal.
Nair , D.N and Padmavathy S. 2014, Impact of Endophytic Microorganisms on Plants, Environment and Humans. Scientific World Journal Volume 2014.
Perkebunan, P. P. d. P., 2014. Varietas Unggul Rami; Litbang Perkebunan. [Online] Available at: http://perkebunan. litbang. pertanian.go.id/varietas-unggul-rami/
Parnidi, & Setyo, B. U. (2003). Keragaman 20 Aksesi Rami (Boehmeria nivea L. Gaudich) Koleksi Balittas di Cobanrondo, Malang. PRosiding Seminar Nasional Hayati, 135–141.
Pan C. L.; Li S. C.; Li Y. J. Factors influencing plant regeneration efficiency of ramie (Boehmeria nivea Gaud). China’s Fiber Crops 17: 1–6; 1995.
Pandey A, Palni LMS, Bag N (2000) Biological hardening of tissue culture raised tea plants through rhizosphere bacteria. Biotechnol Lett 22:1087–1091
Petruszka, M. 1977. Ramie fibre production and manufacture.
Food and Agric Indus. Service, Rome, p. 285.
Pospisilova, J.,Ticha, I., Ek, P. K. C.,& Haisel, D.(1999). Acclimatization of micropropagated plants to ex vitro conditions. Plantarum Biologia, 42(96), 481–497.
Ramamoorthy V, Vishwanathan R, Raguchander T et al (2001) Induction of systematic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Prot 20:1–11
Purwati, R.D dan Sudjindro. Peluang Perbanyakan Bibit Melalui Kultur Jaringan untuk Mendukung Pengembangan Rami. Balitas doc.
Sharma, A. K., Laha, S. K., Sinha, M. K., & Mahapatra, B. S. (2010). Ramie : A fibre crop of new opportunities. Indian Farming.
Senthilkumar M, Madhaiyan M, Sundaram SP et al (2008) Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans. Biotechnol Lett 30:1477–1487
Silvente, S., and Trippi, V. S., 1986, Sucrose-modulated morphogenesis in Anagallis arvensis L., Plant Cell Physiol. 27: 349
Srivastava PS, Bharti N, Pandey D et al (2002) Role of mycorrhiza in in vitro micropropagation of plants. In: Mukerji KG, Manoharachari C, Chamola BP (eds) Techniques in mycorrhizal studies. Kluwer Academic Publishers, The Netherlands, pp 443–460
Sut, D., Talukdar, & Singh, S. (2004). Micro-propagation of ramie. Indian Journal of Agricultural Research, 38(2), 131–134.
Ticha, I., Radochova, B., Kadlecek, P.: Stomatal morphology during acclimatization of tobacco plantlets to ex vitro conditions.- Biol. Plant. 42: 469-474, 1999.
Trivedi, P., & Pandey, A. (2007). Biological hardening of micropropagated Picrorhiza kurrooa Royel ex Benth., an endangered species of medical importance. World Journal of Microbiology and Biotechnology, 23(6), 877–878. https:// doi.org/10.1007/s11274-006-9293-3
Van Huylenbroeck JM, Huygens H, Debergh PC (1995) Photoinhibition during acclimatization of microprogagated Spathiphyllum “Petie” plantlets. In Vitro Cell Dev Biol Plant 31:160–164
Wang, B., Peng, D. X., Sun, Z. X., Zhang, N., & Gao, S. M. (2008). In vitro plant regeneration from seedling-derived explants of ramie [ Boehmeria nivea ( L .) Gaud ], 105–111. https://doi. org/10.1007/s11627-008-9121-6
Wang B, Peng D, Liu L, Sun Z, Zhang N, Gao S. 2007. An efficient adventitious shoot regeneration system for ramie (Boehmeria nivea Gaud) using thidiazuron. Bot Stud 48: 173- 180.
Zhang, T., X.Y. Wang, and Z.Y. Cao. 2005. Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects. Bio. Plant. 49: 423-426.
Zhou, P.H. 1980. Research note on tissue culture of ramie (Boehmeria nivea Gaud). China’s Fiber Crops. 1: 31-32
Downloads
Published
September 25, 2025
HOW TO CITE
Copyright (c) 2021 Asri Peni Wulandari, Anne Nuraini, Nurzaman, dan Rully Dyah Purwati
License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Details about this monograph
ISBN-13 (15)
978-623-224-562-4












