Mitigasi Metan Enterik pada Ruminansia untuk Mewujudkan Peternakan Ramah Lingkungan dan Berkelanjutan

Authors

Roni Ridwan
Badan Riset dan Inovasi Nasional

Keywords:

metan enterik, gas rumah kaca, efisiensi pakan, omik, Produktivitas ternak, Berkelanjutan

Synopsis

Arah kebijakan pemerintah sesuai dengan RPJMN 2025-2029 dibidang pertanian-peternakan adalah meningkatkan produktivitas ternak dan memperkuat ketahanan pangan menuju swasembada pangan hewani nasional (Peraturan Presiden [PP-RI], 2025). Sejalan dengan arah kebijakan tersebut BRIN berkontribusi melalui riset dasar, terapan, dan aplikasi yang lebih memfokuskan pada pemanfaatan sumber daya genetik lokal dengan tool bioteknologi modern secara komprehensif untuk menjawab permasalahan saat ini dan tantangan masa depan. Pada orasi ini disampaikan kebaruan mitigasi metan (CH4) enterik pada ruminansia melalui implementasi hasil riset dari rekayasa pakan, pemberian pakan berbasis mikroba berupa probiotik dan pakan fermentasi silase, dan penambahan pakan imbuhan lainnya berupa polifenol tanin, prebiotik, enzim, asam amino terproteksi, dan asam lemak dengan dosis yang tepat menjadi kunci keberhasilan dalam mewujudkan peternakan yang ramah lingkungan dan berkelanjutan. Mitigasi CH4 enterik pada ruminansia diterapkan melalui rekayasa efisiensi pakan yang memberikan penghambatan secara langsung dan tidak langsung dengan daya cerna pakan, aktivitas interaksi mikrobioma rumen; bakteri, protozoa dan metanogen, dan mengubah pola metabolisme rumen melalui evaluasi pendekatan omik. Dinamika struktur mikrobioma dan profil metabolit menunjukkan dampak positif terhadap produktivitas ruminansia. Hasil ini membuka peluang yang menjanjikan untuk masa depan dan aplikasi praktis dalam manajemen ternak untuk meningkatkan produktivitas yang berkelanjutan.

 

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Author Biography

Roni Ridwan, Badan Riset dan Inovasi Nasional

Roni Ridwan, lahir di Cianjur, Jawa Barat pada tanggal 16 Juli 1975, adalah anak ke tiga (3) dari pasangan Bapak Endang Suanda (Alm) dan Ibu Rohayati Fatimah. Menikah dengan Tika Sundari, S.Pt. dan dikaruniai tiga (3) orang anak, yaitu Kania Dewi Shiyam, S.Si., Muhamad Aqil Arrasyid, dan Aisha Dewi Humaira.

Berdasarkan Keputusan Presiden Republik Indonesia Nomor 2/M Tahun 2023 tanggal 9 Januari 2023 diangkat sebagai Peneliti Ahli Utama terhitung mulai 25 Januari 2023.

Berdasarkan Keputusan Kepala Badan Riset dan Inovasi Nasional Nomor 143/I/HK/2025 tanggal 10 Juni 2025 yang bersangkutan dapat melakukan orasi pengukuhan Profesor Riset.

Menamatkan Sekolah Dasar di SDN Cibogo 1 Desa Ciranjang Kecamatan Ciranjang, Kabupaten Cianjur pada tahun 1988, Sekolah Menengah Pertama di SMPN 1 Ciranjang Kecamatan Ciranjang Kab. Cianjur pada tahun 1991, dan Sekolah Menengah Atas di SMAN 1 Cianjur, Kab. Cianjur pada tahun 1994. Memperoleh gelar Sarjana Ilmu Nutrisi dan Makanan Ternak dari IPB tahun 2000, gelar Magister Bidang Bioteknologi dari IPB tahun 2009, dan gelar Doktor Bidang Mikrobiologi dari IPB tahun 2014.

Mengikuti beberapa pelatihan yang terkait dengan bidang kompetensinya, antara lain: diklat fungsional tingkat dasar tahun 2001 dan lanjutan tahun 2016 di LIPI, Ekologi Molekuler Actinomycetes Indonesia di NITE-Kisarazu Jepang tahun 2006, Ekologi Molekuler Mikroba Rumen sebagai Research Exchange RISTEK RI di RIKEN Jepang tahun 2013, Ekologi Mikroba Rumen di RIKEN Jepang tahun 2015 dan Feed Product Management di Cordoba Spanyol tahun 2016.

Pernah menduduki jabatan struktural sebagai Kasub Bidang Sarana dan Prasarana Biologi Sel dan Jaringan Pusat Penelitian Bioteknologi LIPI (tahun 2011–2012). Jabatan fungsional peneliti diawali sebagai Peneliti Ahli Pertama (III/a) tahun 2002, Peneliti Ahli Pertama (III/b) tahun 2007, Peneliti Ahli Muda (III/c) tahun 2009, Peneliti Ahli Muda (III/d) tahun 2015, Peneliti Ahli Madya (IV/a) tahun 2016, dan memperoleh jabatan Peneliti Ahli Utama (IV/d) bidang Bioteknologi Hewan tahun 2023.

Menghasilkan 104 karya tulis ilmiah (KTI), baik yang ditulis sendiri maupun bersama penulis lain dalam bentuk buku, jurnal, dan prosiding. Sebanyak 87 KTI ditulis dalam bahasa Inggris, dan 17 KTI dalam bahasa Indonesia. Sebanyak 24 kekayaan intelektual dimiliki dalam bentuk 17 paten terdaftar dan 7 paten granted serta sebanyak 1 paten telah dilisensikan.

Ikut serta dalam pembinaan kader ilmiah, yaitu sebagai pembimbing jabatan fungsional peneliti sebanyak 5 orang pada LIPI dan BRIN, pembimbing skripsi (S-1) sebanyak 19 mahasiswa pada IPB, UNPAD, UNES, UNJ, UTS, UP; pembimbing tesis (S-2) sebanyak 19 mahasiswa pada IPB dan UNPAD; pembimbing disertasi (S-3) sebanyak 14 mahasiswa pada IPB, UNPAD, USK, UNAND; serta penguji disertasi (S-5) pada IPB dan UNPAD. Aktif dalam organisasi profesi ilmiah, yaitu sebagai anggota AINI (2019–sekarang), HILPI (2019–sekarang), dan PPI (2019-sekarang). Menerima tanda penghargaan Satyalancana Karya Satya 10 tahun (X) tahun 2015, dan Satyalancana Karya Satya 20 tahun (XX) tahun 2021 dari Presiden RI.

References

Abdelbagi, M., Ridwan, R., Fidriyanto, R., Nayohan, S., & Nahrowi, N. (2024). Effects of encapsulation and combining probiotics with different nitrate forms on methane emission and in vitro rumen fermentation characteristics. Open Agriculture, 9(1):20220377. https://doi.org/10.1515/opag-2022-0377

Abdelbagi, M., Ridwan, R., Fidriyanto, R., Rohmatussolihat, Nahrowi, & Jayanegara, A. (2021). Effects of probiotics and encapsulated probiotics on enteric methane emission and nutrient digestibility in vitro. IOP Conference Series: Earth and Environmental Science, 788(1). https://doi.org/10.1088/1755-1315/788/1/012050

Abdelbagi, M., Ridwan, R., Fitri, A., Nahrowi, & Jayanegarac, A. (2023). Performance, Methane Emission, Nutrient Utilization, and the Nitrate Toxicity of Ruminants with Dietary Nitrate Addition: A Meta-analysis from In Vivo Trials. Tropical Animal Science Journal, 46(1), 74–84. https://doi.org/10.5398/tasj.2023.46.1.74

Abdelbagi, M., Ridwan, R., Nahrowi, & Jayanegara, A. (2021). The potential of nitrate supplementation for modulating the fermentation pattern and mitigating methane emission in ruminants: A meta-analysis from in vitro experiments. IOP Conference Series: Earth and Environmental Science, 902(1). https://doi.org/10.1088/1755-1315/902/1/012023

Adawiah, A., Meryandini, A., Ridwan, R., Fidriyanto, R., & Ageng, K. (2025). The rumen microbiome and metabolome profile of Ongole crossbreed cattle fed probiotics and protected amino acids. Tropical Animal Health and Production 57(148). https://doi.org/10.1007/s11250-025-04400-z

Al-shawi, S. G., Dang, D. S., Yousif, A. Y., Al-younis, Z. K., Najm, T. A., & Matarneh, S. K. (2020). The potential use of probiotics to improve animal health, efficiency, and meat quality : a review. Agriculture, 10(10), 452. https://doi.org/10.3390/agriculture10100452

Anadon, A., Rosa Martinez-Larranaga, M., & Aranzazu Martínez, M. (2006). Probiotics for animal nutrition in the European Union. Regulation and safety assessment. Regulatory Toxicology and Pharmacology, 45(1), 91–95. https://doi.org/10.1016/j.yrtph.2006.02.004

Arisya, W., Ridwan, R., Ridla, M., & Jayanegara, A. (2019). Tannin treatment for protecting feed protein degradation in the rumen in vitro. Journal of Physics: Conference Series, 1360(1), 0–5. https://doi.org/10.1088/1742-6596/1360/1/012022

Astuti, W. D., Wiryawan K. G., Wina, E., Widyastuti, Y., Suharti, S. & Ridwan R. (2018). Pakistan Journal of Nutrition. Pakistan Journal of Nutrition, 17(3), 131–139. https://doi.org/10.3923/pjn.2018.131.139

Astuti, W. D., Ridwan, R., Fidriyanto, R., Rohmatussolihat, R., Sari, N. F., Sarwono, K. A., Fitri, A., & Widyastuti, Y. (2022). Changes in rumen fermentation and bacterial profiles after administering Lactiplantibacillus plantarum as a probiotic. Veterinary World, 15(8), 1969–1974. https://doi.org/10.14202/vetworld.2022.1969-1974

Astuti, W. D., Ridwan, R., & Tappa, B. (2007). Penggunaan Probiotik dan Kromium Organik terhadap Kondisi Lingkungan Rumen In Vitro. JITV, 12(4), 262–267.

Astuti, W. D., Widyastuti, Y., Fidriyanto, R., Ridwan, R., Rohmatussolihat, Sari, N. F., Firsoni, & Sugoro, I. (2020). In vitro gas production and digestibility of oil palm frond silage mixed with different levels of elephant grass. IOP Conference Series: Earth and Environmental Science, 439(1). https://doi.org/10.1088/1755-1315/439/1/012022

Astuti, W. D., Widyastuti, Y., Ridwan, R., & Yetti, E. (2013). Quality of vegetable waste silages treated with various carbohydrate sources. Media Peternakan, 36(2), 120–125. https://doi.org/10.5398/medpet.2013.36.2.120

Astuti, W. D., Widyastuti, Y., Wina, E., Suharti, S., Ridwan, R., & Wiryawan, K. G. (2018). Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR. Journal of the Indonesian Tropical Animal Agriculture, 43(2), 184–192. https://doi.org/10.14710/jitaa.43.2.184-192

Astuti, W., Ridwan, R., & Widyastuti, Y. (2009). Microscopic Observation of Napier Grass (Pennisetum purpureum) Structure: Preliminary Study on the Effects of Rumen Microbial Degradation. Biotechindonesia.Org, 2(1), 1–5. http://www.biotechindonesia.org/journal/jaib/jab-2009-01-02/jab-1-09-7.pdf

Azzahra, F.R, Susanto, I., R. Ridwan, & Jayanegara, A. (2025). Organoleptic and physical quality of silage as affected by the addition of lactic acid bacteria and acacia tannin extract. IOP Conf. Series: Earth and Environmental Science 1484 012006. https://doi.org/10.1088/1755-1315/1484/1/012006

Badan Pangan Nasional [BPN]. (2024). Direktori Perkembangan Konsumsi Pangan Nasional dan Provinsi Tahun 2019-2023.

Badan Pusat Statistik [BPS]. (2024). Statistik Indonesia 2023. Badan Pusat Statistik, 52, 790. https://www.bps.go.id/publication/2020/04/29/e9011b3155d45d70823c141f/statistik-indonesia-2020.html

Bahri dan Tiesnamurti. (2012). Strategi pembangunan peternakan berkelanjutan dengan memanfaatkan sumber daya lokal. Jurnal Penelitian Dan Pengembangan Pertanian, 31(4), 30919. https://doi.org/10.21082/jp3.v31n4.2012.p%p

Beauchemin, K. A., Ungerfeld, E. M., Abdalla, A. L., Alvarez, C., Arndt, C., Becquet, P., Benchaar, C., Berndt, A., Mauricio, R. M., McAllister, T. A., Oyhantçabal, W., Salami, S. A., Shalloo, L., Sun, Y., Tricarico, J., Uwizeye, A., De Camillis, C., Bernoux, M., Robinson, T., & Kebreab, E. (2022). Invited review: Current enteric methane mitigation options. Journal of Dairy Science, 105(12), 9297–9326. https://doi.org/10.3168/jds.2022-22091

Beauchemin, K. A., Ungerfeld, E. M., Eckard, R. J., & Wang, M. (2020). Review: Fifty years of research on rumen methanogenesis: lessons learned and future challenges for mitigation. Animal, The International Journal of Animal Biosciences, 14, s2–s16. https://doi.org/10.1017/S1751731119003100

Bodas, R., Prieto, N., García-González, R., Andrés, S., Giráldez, F. J., & López, S. (2012). Manipulation of rumen fermentation and methane production with plant secondary metabolites. Animal Feed Science and Technology, 176(1–4), 78–93. https://doi.org/10.1016/j.anifeedsci.2012.07.010

Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., & Wittwer, C. T. (2009). The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 55(4), 611–622. https://doi.org/10.1373/clinchem.2008.112797

Capasso, V., Lotito, D., Pugliese, G., Ruocco, R. A., & Musco, N. (2017). Essential Oils and Methanogenesis. A Review. Journal of Nutritional Ecology and Food Research 4:1–21. https://doi.org/10.1166/jnef.2017.1162

Cholewinska, P., Czyz, K., Nowakowski, P., & Wyrostek, A. (2020). The microbiome of the digestive system of ruminants-a review. Animal Health Research Reviews, 21(1), 3–14. https://doi.org/10.1017/S1466252319000069

Demirel, B., & Scherer, P. (2008). The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: A review. Environmental Science and Biotechnology, 7(2), 173–190. https://doi.org/10.1007/s11157-008-9131-1

Ditjen PKH. (2023). Statistics of Food Consumption 2023. Kementerian Pertanian, 1–132. https://satudata.pertanian.go.id/assets/docs/publikasi/Buku_Statsitik_Konsumsi_Pangan_2023.pdf

Doyle, N., Mbandlwa, P., Kelly, W. J., Attwood, G., Li, Y., Ross, R. P., Stanton, C., Leahy, S., Hebert, E. M., & Snelling, T. J. (2019). Use of lactic acid bacteria to reduce methane production in ruminants, a critical review general characteristics of lactic acid. Frontiers in Microbiology 10:2207. https://doi.org/10.3389/fmicb.2019.02207

Du, Z., Nakagawa, A., Fang, J., Ridwan, R., Astuti, W. D., Sarwono, K. A., Sofyan, A., Widyastuti, Y., & Cai, Y. (2024). Cleaner anaerobic fermentation and greenhouse gas reduction of crop straw. Microbiology Spectrum 12(7). https://doi.org/10.1128/spectrum.00520-24

Ellis, J. L., Bannink, A., Hindrichsen, I. K., Kinley, R. D., Pellikaan, W. F., Milora, N., & Dijkstra, J. (2016). The effect of lactic acid bacteria included as a probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Animal Feed Science and Technology, 211, 61–74. https://doi.org/10.1016/j.anifeedsci.2015.10.016

FAO. (2023). Achieving SDG 2 without breaching the 1 . 5 o C threshold : A global roadmap. https://doi.org/https://doi.org/10.4060/cc9113en

Fidriyanto, R., Juanssilfero, A. B., Sarwono, K. A., Ridwan, R., Nahrowi, N., & Jayanegara, A. (2024). Enhancing physicochemical, rheological properties, and in vitro rumen fermentation of starch with Melastoma candidum D. Don fruit extract. Animal Science Journal, 95(1), 1–15. https://doi.org/10.1111/asj.13950

Fidriyanto, R., Priadi, G., Berlinda Paradisa, Y., Dwi Astuti, W., Ridwan, R., Ageng Sarwono, K., Whatman, M., & Widyastuti, Y. (2021). Pengaruh substitusi rumput gajah dengan limbah serai pada pakan ruminansia tinggi hijauan terhadap kecernaan danproduksi metan secara in vitro. Agric, 33(2), 103–114.

Fidriyanto, R., Priadi, G., Ridwan, R., Nahrowi, N., & Jayanegara, A. (2023). Physicochemical, thermal properties, and in vitro rumen fermentation of four different underutilized fruit by-products. Biodiversitas, 24(4), 2416–2425. https://doi.org/10.13057/biodiv/d240457

Fidriyanto, R., Ridwan, R., Nahrowi, & Jayanegara, A. (2023). Utilization of fruits by product as ruminant feed: in vitro digestibility and methane production. AIP Conference Proceedings, 2606. https://doi.org/10.1063/5.0118524

Fidriyanto, R., Ridwan, R., Rohmatussolihat, R., Astuti, W. D., Paradisa, Y. B., Sarwono, K. A., Fitri, A., & Widyastuti, Y. (2023). The ability of Lactiplantibacillus plantarum TSD-10 and DR-162 to reduce aflatoxin and microbial contamination of corn. Agric, 35(1), 1–12. https://doi.org/10.24246/agric.2023.v35.i1.p1-12

Fidriyanto, R., Ridwan, R., Watman, M., Adi, E. B. M., Mulyaningsih, E. S., Jayanegara, A., Astuti, W. D., Rohmatussolihat, Sari, N. F., & Widyastuti, Y. (2021). Evaluation of parboiled rice by-product as a ruminant feed: In vitro digestibility and methane production. IOP Conference Series: Earth and Environmental Science, 762(1). https://doi.org/10.1088/1755-1315/762/1/012045

Fitri, A., Ridwan, R., Astuti, W. D., Fidriyanto, R., Rohmatussolihat, Sarwono, K. A., Widyastuti, Y., Sofyan, A., Yanza, Y. R., Wardani, W. W., Sanoesi, T. B. T., Putra, A. A., & Jayanegara, A. (2023). Effect of rumen-protected methionine on rumen fermentation and plasma amino acid concentration on ruminants: A meta-analysis. AIP Conf. Proc. 2972, 020002-1–020002-5;, 020002. https://doi.org/10.1063/5.0183631

Fitri, A., Yanza, Y. R., Jayanegara, A., Ridwan, R., Astuti, W. D., Sarwono, K. A., Fidriyanto, R., Rohmatussolihat, R., Widyastuti, Y., & Obitsu, T. (2022). Divergence effects between dietary Acacia and Quebracho tannin extracts on nutrient utilization, performance, and methane emission of ruminants: A meta-analysis. Animal Science Journal, 93(1), 1–13. https://doi.org/10.1111/asj.13765

Fuller. (1989). Probiotics in man and animals. Journal of Applied Bacteriology, 66, 365–378.

Hartina, W. A., Ridwan, R., Diapari, D., Fidriyanto, R., & Jayanegara, A. (2021). The addition of feed additive in beef cattle ration on in vitro fermentation characteristics. IOP Conference Series: Earth and Environmental Science, 888(1). https://doi.org/10.1088/1755-1315/888/1/012074

Holtzapple, M. T., Wu, H., Weimer, P. J., Dalke, R., Granda, C. B., Mai, J., & Urgun-Demirtas, M. (2022). Microbial communities for valorizing biomass using the carboxylate platform to produce volatile fatty acids: A review. Bioresource Technology, 344,126253. https://doi.org/10.1016/j.biortech.2021.126253

Huang, Q., Liu, X., Zhao, G., Hu, T., & Wang, Y. (2018). Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production. Animal Nutrition, 4(2), 137–150. https://doi.org/10.1016/j.aninu.2017.09.004

Ikusika, O. O., Haruzivi, C., & Mpendulo, T. (2022). Alternatives to the use of antibiotics in animal production. Intech Open, 59(10), 118–131.

Intergovernmental Panel on Climate Change [IPCC]. (2023). Climate Change 2022 - Mitigation of Climate Change. https://doi.org/10.1017/9781009157926

Irawan, A., Sofyan, A., Ridwan, R., Hassim, H. A., Respati, A. N., Wardani, W. W., Sadarman, Astuti, W. D., & Jayanegara, A. (2021). Effects of different lactic acid bacteria groups and fibrolytic enzymes as additives on silage quality: A meta-analysis. Bioresource Technology Reports, 14, 100654. https://doi.org/10.1016/j.biteb.2021.100654

Irawan, A., Sofyan, A., Wahyono, T., Harahap, M. A., Febrisiantosa, A., Sakti, A. A., Herdian, H., & Jayanegara, A. (2023). Relationships between dietary rumen-protected lysine and methionine with the lactational performance of dairy cows — A meta-analysis. Animal Bioscience, 36(11), 1666–1684. https://doi.org/10.5713/ab.23.0084

Islami, I., Evvyernie, D., Rohmatussolihat, Astuti, W. D., Fitri, A., Fidriyanto, R., Sarwono, K. A., Widyastuti, Y., & Ridwan, R. (2024). The addition of probiotic in selected production media and enzymes on rumen fermentability characteristics. IOP Conference Series: Earth and Environmental Science, 1359(1), 2–7. https://doi.org/10.1088/1755-1315/1359/1/012115

Jayanegara, A., Ardhisty, N. F., Dewi, S., Antonius, A., Ridwan, R., Laconi, E. B., Nahrowi, N., & Ridla, M. (2019). Enhancing nutritional quality of oil palm empty fruit bunch for animal feed by using fiber cracking technology. Advances in Animal and Veterinary Sciences, 7(3), 157–163. https://doi.org/10.17582/journal.aavs/2019/7.3.157.163

Jayanegara, A., Gustanti, R., Ridwan, R., & Widyastuti, Y. (2020). Fatty acid profiles of some insect oils and their effects on in vitro bovine rumen fermentation and methanogenesis. Italian Journal of Animal Science, 19(1), 1311–1318. https://doi.org/10.1080/1828051X.2020.1841571

Jayanegara, A., Sari, Y. C., Ridwan, R., Diapari, D., & Laconi, E. B. (2017). Protein fractionation and utilization of soybean and redbean as affected by different drying temperature. Buletin Peternakan, 41(1), 37. https://doi.org/10.21059/buletinpeternak.v41i1.13922

Jayanegara, A., Wina, E., Soliva, C. R., Marquardt, S., Kreuzer, M., & Leiber, F. (2011). Dependence of forage quality and methanogenic potential of tropical plants on their phenolic fractions as determined by principal component analysis. Animal Feed Science and Technology, 163(2–4), 231–243. https://doi.org/10.1016/j.anifeedsci.2010.11.009

Jayanegara, A., Wina, E., & Takahashi, J. (2014). Meta-analysis on methane mitigating properties of saponin-rich sources in the rumen : influence of addition levels and plant sources. 27(10), 1426–1435.

Ji, E. S., & Park, K. H. (2012). Methane and nitrous oxide emissions from livestock agriculture in 16 local administrative districts of korea. Asian-Australasian Journal of Animal Sciences, 25(12), 1768–1774. https://doi.org/10.5713/ajas.2012.12418

Jiang, B., Qin, C., Xu, Y., Song, X., Fu, Y., Li, R., Liu, Q., & Shi, D. (2024). Multi-omics reveals the mechanism of rumen microbiome and its metabolome together with host metabolome participating in the regulation of milk production traits in dairy buffaloes. Front. Microbiol 15:1–22. https://doi.org/10.3389/fmicb.2024.1301292

Johnson, K. A., & Johnson, D. E. (1995). Methane emissions from cattle. Journal of Animal Science, 73(8), 2483–2492. https://doi.org/10.2527/1995.7382483x

Kementerian Lingkungan Hidup dan Kehutanan [KLHK]. (2021). Indonesia Third Biennial Update Report. Republic of Indonesia, 66.

Khairunisa, B. H., Heryakusuma, C., Ike, K., Mukhopadhyay, B., & Susanti, D. (2023). Evolving understanding of rumen methanogen ecophysiology. Frontiers in Microbiology, 14(November). https://doi.org/10.3389/fmicb.2023.1296008

Khan, F. A., Ali, A., Wu, D., Huang, C., Zulfiqar, H., Ali, M., Ahmed, B., Yousaf, M. R., Putri, E. M., Negara, W., Imran, M., & Pandupuspitasari, N. S. (2024). Editing microbes to mitigate enteric methane emissions in livestock. World Journal of Microbiology and Biotechnology, 40(10), 1–17. https://doi.org/10.1007/s11274-024-04103-x

Khan, F. A., Pandupuspitasari, N. S., Huang, C., Negara, W., Ahmed, B., Putri, E. M., Lestari, P., Priyatno, T. P., Prima, A., Restitrisnani, V., Surachman, M., Akhadiarto, S., Darmawan, I. W. A., Wahyuni, D. S., & Herdis, H. (2023). Unlocking gut microbiota potential of dairy cows in varied environmental conditions using shotgun metagenomic approach. BMC Microbiology, 23(1), 1–11. https://doi.org/10.1186/s12866-023-03101-7

Komalasari, K., Astuti, D. A., Widyastuti, Y., Astuti, W. D., & Ridwan, R. (2014). Rumen fermentation and milk quality of dairy cows fed complete feed silages. Media Peternakan, 37(1), 38–42. https://doi.org/10.5398/medpet.2014.37.1.38

Kroliczewska, B., Pecka-Kielb, E., & Bujok,J. (2023). Strategies used to reduce methane emissions from ruminants: controversies and issues. Agriculture (Switzerland), 13(3). https://doi.org/10.3390/agriculture13030602

Ku-Vera, J. C., Jimenez-Ocampo, R., Valencia-Salazar, S. S., Montoya-Flores, M. D., Molina-Botero, I. C., Arango, J., Gomez-Bravo, C. A., Aguilar-Perez, C. F., & Solorio-Sanchez, F. J. (2020). Role of secondary plant metabolites on enteric methane mitigation in ruminants. Frontiers in Veterinary Science, 7, 1–14. https://doi.org/10.3389/fvets.2020.00584

Makmur, M., Yanza, Y. R., Fitri, A., Syarifuddin, Ridwan, R., & Jayanegara, A. (2023). Effects of essential oils and their derivatives on rumen fermentation characteristics and pufa biohydrogenation: A Meta-Analysis of In Vitro Studies. Veterinary Integrative Sciences, 21(3), 925–944. https://doi.org/10.12982/VIS.2023.067

Matthews, C., Crispie, F., Lewis, E., Reid, M., O’Toole, P. W., & Cotter, P. D. (2019). The rumen microbiome: a crucial consideration when optimising milk and meat production and nitrogen utilisation efficiency. Gut Microbes, 10(2), 115–132. https://doi.org/10.1080/19490976.2018.1505176

McAllister, T. A., Beauchemin, K. A., Alazzeh, A. Y., Baah, J., Teather, R. M., & Stanford, K. (2011). Review: The use of direct fed microbials to mitigate pathogens and enhance production in cattle. Canadian Journal of Animal Science, 91(2), 193–211. https://doi.org/10.4141/cjas10047

Morgavi, D. P., Forano, E., Martin, C., & Newbold, C. J. (2010). Microbial ecosystem and methanogenesis in ruminants. Animal, 4(7), 1024–1036. https://doi.org/10.1017/S1751731110000546

Moss, A. R., Jouany, J. P., & Newbold, J. (2000). Methane production by ruminants: Its contribution to global warming. Animal Research, 49(3), 231–253. https://doi.org/10.1051/animres:2000119

Mulianda, R., Ridla, M., Laconi, E. B., Ridwan, R., & Jayanegara, A. (2019). Comparison of nutritive value between intact and defatted black soldier fly larvae for animal feed. IOP Conference Series: Materials Science and Engineering, 546(4). https://doi.org/10.1088/1757-899X/546/4/042024

Muthia, D. Ridlla, M. Laconi, E.B. Ridwan, R. Fidriyanto, R. Abdelbagi, M. Harahap, R. P. Jayanegara, A. (2021). Effects of Ensiling, Urea Treatment and Autoclaving on Nutritive Value and In-vitro Rumen Fermentation of Rice Straw. Advances in Animal and Veterinary Sciences, 9(5). http://dx.doi.org/10.17582/journal.aavs/2021/9.5.655.661

Muthia, D., Laconi, E. B., Ridla, M., Jayanegara, A., Ridwan, R., Fidriyanto, R., Abdelbagi, M., & Ramdani, H. (2021). Effect of combining autoclave and ammoniation on nutritional value and in vitro digestibility of rice straw. IOP Conference Series: Earth and Environmental Science, 788(1). https://doi.org/10.1088/1755-1315/788/1/012052

Negara, W., Roswanjaya, Y. P., Parastiwi, H. A., Purba, R. D., Martono, S., Maulana, S., Gopar, R. A., Negoro, P. S., Wahyuni, D. S., Surachman, M., Darmawan, W. A., Rofiq, N., Akhadiarto, S., Widiawati, Y., & Ridwan, R. (2024). The development of probiotics for reducing methane gas emission in indonesian local beef cattle: in vitro assays. AIP Conference Proceedings, 2957(1). https://doi.org/10.1063/5.0184285

Niehaus, A. J. (2008). Rumenotomy. Veterinary Clinics of North America - Food Animal Practice, 24(2), 341–347. https://doi.org/10.1016/j.cvfa.2008.02.011

Nurfitriani, R. A., Jayanegara, A., Kumalasari, N. R., Ratnakomala, S., Rohmatussolihat, Sari, N. F., Fidriyanto, R., Sarwono, K. A., Astuti, W. D., Fitri, A., Widyastuti, Y., & Ridwan, R. (2023). Effects of bionanomineral selenium (BioNano-Se) and probiotics inclusion to ration on in vitro rumen fermentation characteristics. Journal of Animal and Plant Sciences, 33(1), 67–74. https://doi.org/10.36899/JAPS.2023.1.0595

Nurfitriani, R. A., Ridwan, R., Jayanegara, A., Kumalasari, N. R., Ratnakomala, S., & Widyastuti, Y. (2020). Produksi bionanomineral selenium dari berbagai jenis strain bakteri asam laktat (BAL) sebagai aditif pada ransum ternak ruminansia. Jurnal Ilmu Peternakan Terapan, 4(1), 20–26. https://doi.org/10.25047/jipt.v4i1.2340

Parastiwi, H. A., Lestari, N. S. H., Yanza, Y. R., Niderkorn, V., Ridwan, R., & Jayanegara, A. (2023). Estimating nutrient composition and polyphenol concentration using Near-Infrared Spectroscopy (NIRS) in tropical forages. Biodiversitas, 24(12), 6652–6660. https://doi.org/10.13057/biodiv/d241227

Patra, A. K. (2014). Trends and projected estimates of GHG emissions from indian livestock in comparisons with GHG emissions from world and developing countries. Asian-Australasian Journal of Animal Sciences, 27(4), 592–599. https://doi.org/10.5713/ajas.2013.13342

Patra, A. K., & Yu, Z. (2012). Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Appl Environ Microbiol. 78(12), 4271–4280. https://doi.org/10.1128/AEM.00309-12

Patra, A., Park, T., Kim, M., & Yu, Z. (2017). Rumen methanogens and mitigation of methane emission by anti-methanogenic compounds and substances. Journal of Animal Science and Biotechnology, 8(1), 1–18. https://doi.org/10.1186/s40104-017-0145-9

Peraturan Presiden [PP-RI]. (2025). Rencana Pembangunan Jangka Menengah Nasional Tahun 2025-2029. Peraturan Presiden [PP-RI].

Putra, L. O., Suharti, S., Sarwono, K. A., Sutikno, S., Fitri, A., Astuti, W. D., Rohmatussolihat, R., Widyastuti, Y., Ridwan, R., Fidriyanto, R., & Wiryawanv, K. G. (2023). The effects of heat-moisture treatment on resistant starch levels in cassava and on fermentation, methanogenesis, and microbial populations in ruminants. Veterinary World, 16, 811–819. https://doi.org/10.14202/vetworld.2023.811-819

Putri, F.J., Sidiq, F., Ridwan, R., dan Widyastuti, Y. (2013). Kecernaan bahan kering dan bahan organik silase campuran pennisetum purpureum dan acacia villosa secara in vitro. Prosiding Seminar Nasional Dan Forum Komunikasi Industri Peternakan, 62, 400–408.

Rabetafika, H. N., Razafindralambo, A., Ebenso, B., & Razafindralambo, H. L. (2023). Probiotics as antibiotic alternatives for human and animal applications. Encyclopedia, 3(2), 561–581. https://doi.org/10.3390/encyclopedia3020040

Ratnakomala, S., Ridwan, R., Kartina, G., & Widyastuti, Y. (2006). The effect of Lactobacillus plantarum 1A-2 and 1BL-2 inoculant on the quality of napier grass silage. Biodiversitas Journal of Biological Diversity, 7(2), 131–134. https://doi.org/10.13057/biodiv/d070208

Ridwan, R., Abdelbagi, M., Sofyan, A., Fidriyanto, R., Astuti, W. D., Fitri, A., Sholikin, M. M., Rohmatussolihat, Sarwono, K. A., Jayanegara, A., & Widyastuti, Y. (2023). A meta-analysis to observe silage microbiome differentiated by the use of inoculant and type of raw material. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1063333

Ridwan, R., Bungsu, A. A. W. A., Astuti, W. D., Rohmatussolihat, R., Sari, N. F., Fidriyanto, R., Jayanegara, A., Wijayanti, I., & Widyastuti, Y. (2018). The use of lactic acid bacteria as ruminant probiotic candidates based on in vitro rumen fermentation characteristics. Buletin Peternakan, 42(1), 31–36. https://doi.org/10.21059/buletinpeternak.v42i1.23317

Ridwan, R., Ratnakomala, S., Kartina, G., & Widyastuti, Y. (2005). Pengaruh penambahan dedak padi dan Lactobacillus plantarum 1BL-2 dalam pembuatan silase rumput gajah (Pennisetum purpureum). Media Peternakan, 28(3).

Ridwan, R., Rusmana, I., Widyastuti, Y., Wiryawan, K. G., Prasetya, B., Sakamoto, M., & Ohkuma, M. (2014). Methane mitigation and microbial diversity of silage diets containing Calliandra calothyrsus in a rumen in Vitro fermentation system. Media Peternakan, 37(2), 121–128. https://doi.org/10.5398/medpet.2014.37.2.121

Ridwan, R., Rusmana, I., Widyastuti, Y., Wiryawan, K. G., Prasetya, B., Sakamoto, M., & Ohkuma, M. (2015). Fermentation characteristics and microbial diversity of tropical grass-legumes silages. Asian-Australasian Journal of Animal Sciences, 28(4), 511–518. https://doi.org/10.5713/ajas.14.0622

Ridwan, R., Rusmana, I., Widyastuti, Y., Wiryawan, K. G., Prasetya, B., Sakamoto, M., & Ohkuma, M. (2019). Bacteria and methanogen community in the rumen fed different levels of grass-legume silages. Biodiversitas, 20(4), 1055–1062. https://doi.org/10.13057/biodiv/d200417

Ridwan, R., Widyastuti, Y., Budiarti, S., & Dinoto, A. (2009). Analysis of rumen microbial population of cattle given silage and probiotics using terminal restriction fragment length polymorphism. Microbiology Indonesia, 3(3), 126–132. https://doi.org/10.5454/mi.3.3.2

Rira, M., Morgavi, D. P., Popova, M., Maxin, G., & Doreau, M. (2022). Microbial colonisation of tannin-rich tropical plants: Interplay between degradability, methane production and tannin disappearance in the rumen. Animal, 16(8), 100589. https://doi.org/10.1016/j.animal.2022.100589

Rohmatussolihat, Ridwan, R., Astuti, W. D., Fidriyanto, R., Meliah, S., Sarwono, K. A., Fitri, A., Sopiani, P., Ragamustari, S. K., Ilyas, M., Widyastuti, Y., Ridla, M., Mubarik, N. R., & Jayanegara, A. (2024). Screening of lactic acid bacteria for silage inoculant candidate. IOP Conference Series: Earth and Environmental Science, 1377(1). https://doi.org/10.1088/1755-1315/1377/1/012082

Rohmatussolihat, Ridwan, R., Sari, N. F., Fidriyanto, R., Astuti, W. D., & Widyastuti, Y. (2020). Probiotic powder production for cattle by using response surface methodology. IOP Conference Series: Earth and Environmental Science, 591(1). https://doi.org/10.1088/1755-1315/591/1/012028

Rohmatussolihat, Ridwan, R., Widyastuti, Y., Sari, N. F., Fidryanto, R., & Astuti, W. D. (2021). Optimization of medium composition for probiotic powder inoculum using the response surface methodology. IOP Conference Series: Earth and Environmental Science, 788(1). https://doi.org/10.1088/1755-1315/788/1/012038

Roques, S., Martinez-Fernandez, G., Ramayo-Caldas, Y., Popova, M., Denman, S., Meale, S. J., & Morgavi, D. P. (2024). Annual review of animal biosciences recent advances in enteric methane mitigation and the long road to sustainable ruminant production. Annual Review of Animal Biosciences, 12, 321–343. https://doi.org/10.1146/annurev-animal-021022-024931

Sadarman, Irawan, A., Ridla, M., Jayanegara, A., Nahrowi, Ridwan, R., Sofyan, A., Herdian, H., darma, I. N. G., wahyono, T., Febrina, D., Harahap, R. P., Nurfitriani, R. A., & Adli, D. N. (2021). Influence of ensiling and tannins addition on rumen degradation kinetics of soy sauce residues. Advances in Animal and Veterinary Sciences, 10(2), 270–276. https://doi.org/10.17582/JOURNAL.AAVS/2022/10.2.270.276

Sadarman, S., Ridla, M., Nahrowi, N., Ridwan, R., Harahap, R. P., Nurfitriani, R. A., & Jayanegara, A. (2019). Kualitas fisik silase ampas kecap dengan aditif tanin akasia (Acacia mangium Wild.) dan aditif lainnya. Jurnal Peternakan, 16(2), 66. https://doi.org/10.24014/jupet.v16i2.7418

Sadarman, S., Ridla, M., Nahrowi, N., Ridwan, R., & Jayanegara, A. (2020). Evaluation of ensiled soy sauce by-product combined with several additives as an animal feed. Veterinary World, 13(5), 940–946. https://doi.org/10.14202/vetworld.2020.940-946

Sagala, Y. G., Andadari, L., Handayani, T. H., Sholikin, M. M., Fitri, A., Fidriyanto, R., Rohmatussolihat, R., Ridwan, R., Astuti, W. D., Widyastuti, Y., Fassah, D. M., Wijayanti, I., & Sarwono, K. A. (2024). The effect of silkworms (Bombyx mori) chitosan on rumen fermentation, methanogenesis, and microbial population in vitro. Veterinary World, 17(6), 1216–1226. https://doi.org/10.14202/vetworld.2024.1216-1226

Saini, P., Ayyanna, R., Kumar, R., Bhowmick, S. K., Bhaskar, V., & Dey, B. (2024). Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria. Frontiers in Microbiology, 15, 1–20. https://doi.org/10.3389/fmicb.2024.1428808

Sari, N. F., Ridwan, R., Rohmatussolihat, Fidriyanto, R., Astuti, W. D., & Widyastuti, Y. (2019). The effect of probiotics on high fiber diet in rumen fermentation characteristics. IOP Conference Series: Earth and Environmental Science, 251(1). https://doi.org/10.1088/1755-1315/251/1/012057

Sari, N. F., Ridwan, R., & Widyastuti, Y. (2017). The quality of corn silage product from technopark of Banyumulek Lombok, West Nusa Tenggara. Buletin Peternakan, 41(2), 156. https://doi.org/10.21059/buletinpeternak.v41i2.15513

Sarwono, K. A., Rohmatussolihat, R., Watman, M., Ratnakomala, S., Astuti, W. D., Fidriyanto, R., Ridwan, R., & Widyastuti, Y. (2022). Characteristics of fresh rice straw silage quality prepared with addition of lactic acid bacteria and crude cellulase. AIMS Agriculture and Food, 7(3), 481–499. https://doi.org/10.3934/agrfood.2022030

Sato, Y., Sato, R., Fukui, E., & Yoshizawa, F. (2024). Impact of rumen microbiome on cattle carcass traits. Scientific Reports, 14(1), 1–11. https://doi.org/10.1038/s41598-024-56603-3

Sidiq, F., Widyastuti, Y., Putri, F. J. & Ridwan R. (2013). Pengaruh taraf inklusi legum acacia villosa terhadap kualitas silase gabungan rumput legum yang diberi aditif berupa Lactobacillus plantarum 1A-2 dan dedak padi. Prosiding Seminar Nasional Dan Forum Komunikasi Industri Peternakan, 450–468.

Stewart, C.S., Flint. H. J. & Bryant, M. P. (1997). The rumen bacteria. In Hobson P. N. & Stewart C. S. (Eds.), The Rumen Microbial Ecosystem. Springer Netherlands. https://doi.org/10.1007/978-94-009-1453-7

Sujarnoko, T. U. P., Jayanegara, A., Ridwan, R., & Nahrowi. (2020). Tannin characteristic from Hevea brasiliensis and Durio zibethinus with pressure and hot water extraction. IOP Conference Series: Earth and Environmental Science, 462(1). https://doi.org/10.1088/1755-1315/462/1/012010

Sujarnoko, T. U. P., Ridwan, R., Nahrowi, & Jayanegara, A. (2020). Extraction of tannin from acacia (Acacia mangium) bark and its use as a feed additive for protecting in vitro ruminal degradation of tofu dregs. Advances in Animal and Veterinary Sciences, 8(7), 761–765. https://doi.org/10.17582/journal.aavs/2020/8.7.761.765

Susanto, I., Mahendra, F. R., Rahmadani, M., Ridwan, R & Jayanegara, A. (2025). Potential of cumin essential oil as inhibitor of deamination during ensiling process : A meta- analysis and in-silico approach. BIO Web of Conferences 155(10008). https://doi.org/10.1051/bioconf/202515510008

Tajima, K., Aminov, R. I., Nagamine, T., Matsui, H., Nakamura, M., & Benno, Y. (2001). Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Applied and Environmental Microbiology, 67(6), 2766–2774. https://doi.org/10.1128/AEM.67.6.2766-2774.2001

Talapessy, C. D., Rahayuningsih, M., Fidriyanto, R., Fitri, A., & Ridwan, R. (2024). Daya cerna dan karakteristik fermentasi rumen dengan penambahan asam amino terenkapsulasi secara in vitro. Jurnal Ilmu Nutrisi dan Teknologi Pakan, 22(2), 85–90. https://doi.org/10.29244/jintp.22.2.85-90

Tapio, I., Snelling, T. J., Strozzi, F., & Wallace, R. J. (2017). The ruminal microbiome associated with methane emissions from ruminant livestock. Journal of Animal Science and Biotechnology, 1–11. https://doi.org/10.1186/s40104-017-0141-0

Tseten, T., Sanjorjo, R. A., Kwon, M., & Kim, S. W. (2022). Strategies to mitigate enteric methane emissions from ruminant animals. Journal of Microbiology and Biotechnology, 32(3), 269–277. https://doi.org/10.4014/jmb.2202.02019

Wafiyyatunnufus, Rahayuningsih, M., Ridwan, R., Fidriyanto, R., Rohmatussolihat, Sarwono, K. A., Astuti, W. D., Ratnakomala, S., Goel, G., & Widyastuti, Y. (2023). Characteristics of chemical hydrolysis pretreatment and cellulolytic enzymes in the processing of oil palm frond waste as a source of fiber for ruminant feed. AIP Conference Proceedings, 2628. https://doi.org/10.1063/5.0154001

Wahyuni, D. S., Jayanegara, A., Wiryawan, K. G., Ridwan, R., Kusumaningrum, S., Akhadiarto, S., Fidriyanto, R., Fitri, A., Darmawan, W. A., Surachman, M., Herdis, H., Gazali, M., Mawarni, G. K., Khan, F. A., & Lideman. (2023). Evaluation of several macroalgae species on methane emission and antioxidant activity based on in vitro rumen fermentation characteristics. IOP Conference Series: Earth and Environmental Science, 1266(1). https://doi.org/10.1088/1755-1315/1266/1/012072

Wang, G. Y., Qin, S. L., Zheng, Y. N., Geng, H. J., Chen, L., Yao, J. H., & Deng, L. (2023). Propionate promotes gluconeogenesis by regulating mechanistic target of rapamycin (mTOR) pathway in calf hepatocytes. Animal Nutrition, 15, 88–98. https://doi.org/10.1016/j.aninu.2023.07.001

Wang, M., Zhang, L., Jiang, X., Wu, S., Yao, J., & Liu, H. (2024). Multiomics analysis revealed that the metabolite profile of raw milk is associated with the lactation stage of dairy cows and could be affected by variations in the ruminal microbiota. Journal of Dairy Science, 107(10), 8709–8721. https://doi.org/10.3168/jds.2024-24753

Waters, S. M., Roskam, E., Smith, P. E., Kenny, D. A., Popova, M., & Eugène, M. (2024). The role of rumen microbiome in the development of methane mitigation strategies for ruminant livestock. Journal of Dairy Science. https://doi.org/10.3168/jds.2024-25778

Widiawati, Y., Tiesnamurti, B., Hidayat, C., Nurhayati, I. S., Wahyono, T., Krisnan, R., Rofiq, M. N., Shiddique, M. I., Ramadhan, B. A., Krishna, N. H., Anggraeny, Y. N., Ginting, S. P., & Munawaroh, I. S. (2019). Emisi Gas Rumah Kaca dari Peternakan di Indonesia dengan TIER 2 IPCC. LIPI Press. https://doi.org/10.55981/brin.461

Widiawati, Y, Shiddieq M. I., Rohaeni, E. S., Anggraeny, Y. N., Firsoni, Sasongko, W. T., Setiasih, Antonius, Hadiatry, M. C., Wardi, Herliatika, A. Widodo, S., Asmairicen, S., Bansi, H., Puspito, S., Riyanti, S., Andreas, E. M. W., Widaringsih, W., & Miraya, N. (2023). Sistem pemeliharaan ternak ruminansia yang adaptif terhadap perubahan iklim. Teknologi Dan Kearifan Lokal Untuk Adaptasi Perubahan Iklim, 203–231. https://doi.org/10.55981/brin.901.c723

Widiawati, Y., Rofiq, M. N., & Tiesnamurti, B. (2016). Methane emission factors for enteric fermentation in beef cattle using IPCC Tier-2 method in Indonesia. Jurnal Ilmu Ternak Dan Veteriner, 21(2), 101. https://doi.org/10.14334/jitv.v21i2.1358

Zaenal, Zulkharnaim, Ridwan, R., Widyastuti, Y., & Syamsu, J. A. (2023). Organoleptic and pH characteristics of corn straw silage with addition of lactic acid bacteria inoculants as beef cattle feed. AIP Conference Proceedings, 2628. https://doi.org/10.1063/5.0144023

Zhao, Y., Tan, J., Fang, L., & Jiang, L. (2024). Science of the Total Environment Harnessing meta-omics to unveil and mitigate methane emissions in ruminants: Integrative approaches and future directions. Science of The Total Environment 951. https://doi.org/10.1016/j.scitotenv.2024.175732

Zoetendal, E. G., Collier, C. T., Koike, S., Mackie, R. I., & Gaskins, H. R. (2004). Molecular ecological analysis of the gastrointestinal microbiota: a review. Journal of Nutrition, 134(2), 465–472. https://doi.org/10.1093/jn/134.2.465

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