Inovasi Produk Pakan Sapi Potong Berbasis Bakteri Asam Laktat untuk Mendukung Usaha Peternakan Nasional

Authors

Yantyati Widyastuti
Research Center for Biotechnology
DOI: https://doi.org/10.14203/press.412

Keywords:

Inovasi Produk, Pakan Sapi Potong, Bakteri Asam Laktat, Peternakan Nasional

Synopsis

Budi daya sapi potong sangat tergantung pada ketersediaan pakan, di mana biaya pakan mencapai 70% pada tata kelola pemeliharaan ternak. Secara global, budi daya sapi potong telah mengalami perubahan, terutama pada manajemen pemberian pakannya. Pada dasarnya, pakan yang dikonsumsi ditujukan untuk memenuhi kebutuhan hidup pokok dan produktivitasnya. Oleh karena itu, strategi global pada pemberian pakan telah diupayakan juga untuk dapat menurunkan produksi gas metana. Salah satu pendekatan yang telah dilakukan adalah menggunakan bakteri asam laktat yang memproduksi asam laktat dan berperan pada fermentasi pakan, serta memengaruhi keseimbangan mikroorganisme pada saluran pencernaan. Dengan konsep yang serupa, yaitu dengan pengaruh probiotik pada kesehatan manu-sia5 maka bakteri asam laktat juga dapat menyehatkan ternak. Peran bakteri asam laktat untuk menurunkan produksi gas metana merupakan konsep global inovasi. Mendukung upaya untuk meningkatkan produktivitas sapi potong maka serangkaian riset mengenai pengembangan pakan sapi potong yang mengandung bakteri asam laktat telah dilakukan di Pusat Penelitian Bioteknologi. Dengan bekal ilmu nutrisi ternak dari pendidikan S1, ditambah dengan mikrobiologi rumen yang diperoleh melalui training Bioteknologi pada awal karier sebagai peneliti, dan ilmu yang diperoleh dari pendidikan S3, telah menjadikan bekal awal yang cukup untuk menjadi peneliti. Kesempatan mengikuti post doc juga telah menambah pengalaman yang sangat berharga untuk dikembangkan di tanah air. Selain itu, berbagai kerja sama riset bidang mikrobiologi juga telah memberikan tambahan ilmu dan pengalaman untuk mampu melakukan riset pakan berbasis bakteri asam laktat bersama tim.

Downloads

Download data is not yet available.

Author Biography

Yantyati Widyastuti, Research Center for Biotechnology

Menghasilkan 101 karya tulis ilmiah (KTI), baik yang di-tulis sendiri maupun dengan penulis lain dalam bentuk makalah yang diterbitkan dalam buku, jurnal, dan prosiding. Sebanyak 73 di antaranya ditulis dalam bahasa Inggris. Paten yang disusun bersama tim sebanyak tujuh buah.

Ikut serta dalam pembinaan kader ilmiah sebagai pem-bimbing dan penguji skripsi (S1), tesis (S2), dan disertasi (S3) pada Institut Pertanian Bogor, Institut Teknologi Bandung, Universitas Indonesia, Universitas Padjadjaran, Universitas Gadjah Mada, Universitas Sebelas Maret, dan Institut Sains dan Teknologi Nasional. Selain itu, aktif dalam organisasi profesi ilmiah sebagai pengurus HILPI dan anggota HIMPENINDO, AINI, serta PERMI.

Menerima tanda penghargaan Satyalancana Karya Satya X tahun (1998), Satyalancana Karya Satya XX tahun (2005), dan Satyalancana Karya Satya XXX tahun (2015) dari Presiden Republik Indonesia.

References

Ramadhany AD, Ermansyah L (penyunting). Statistik peternakan dan kesehatan hewan 2020. Jakarta: Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementerian Pertanian RI; 2020. https://ditjenpkh.pertanian.go.id

Moss AR, Jouany JP, Newbold J. Methane production by ruminants: Its contribution to global warming. Annales de Zootechnie 2000; 49: 231–253.

Doyle N, Mbandlwa P, Kelly WJ, Attwood G, Li Y, Ross RP, Stanton C, Leahy S. Use of lactic acid bacteria to reduce methane production in ruminants, a critical review. Frontiers in Microbiology 2019; 10: 2207.

Ellis JL, Hindrichsen IK, Klop G, Kinley RD, Milora N, Bannink A, Dijkstra J. Effects of lactic acid bacteria silage inoculation on methane emission and productivity of Holstein Friesian dairy cattle. Journal of Dairy Science. 2016; 99: 7159–7174.

Tannock GW. A Special fondness for lactobacilli. Applied and Environmental Microbiology 2004; 70(6): 3189–3194.

Bolsen KK, Ashbell G, Wilkinson JM. Silage additives. Dalam: Biotechnology in animal feeds and animal feeding. Weinheim: VCH; 1995. 33–54.

Widyastuti Y. Fermentasi silase dan manfaat probiotik silase bagi ruminansia. Media Peternakan 2008; 31(3): 225–232.

Weinberg ZG, Chen Y, Gamburg M. The passage of lactic acid bacteria from silage into rumen fluid, in vitro studies. Journal of Dairy Science 2004; 87: 3386–3397.

Han H, Ogata Y, Yamamoto Y, Nagao S, Nishino N. Identification of lactic acid bacteria in the rumen and feces of dairy cows fed total mixed ration silage to assess the survival of silage bacteria in the gut. Journal of Dairy Science 2014; 97: 5754–5762.

Huyen NT, Martinez I, Pellikaan W. Using lactic acid bacteria as silage inoculants or direct-fed microbials to improve in vitro degradability and reduce methane emissions in dairy cows. Agronomy 2020; 10: 1482. doi:10.3390/agronomy10101482.

Badan Standardisasi Nasional. Pakan konsentrat–Bagian 1: Sapi perah. Standar Nasional Indonesia. 2017.

Badan Standardisasi Nasional. Pakan konsentrat–Bagian 2: Sapi potong. Standar Nasional Indonesia. 2017.

Peraturan Pemerintah Republik Indonesia Nomor 21 Tahun 2005 tentang Keamanan Hayati Produk Rekayasa Genetik. 18 Hal.

Tiesnamurti B, Amirhusin B, Muharsini S, Purwantari ND, Sartika T, Widiawati Y, Trijatmiko KR, Sutoro, Widyastuti Y, Sudarsono, Soejoedono RD, Imron, Ath-Thar MHF. Pedoman pengkajian keamanan pakan produk rekayasa genetik. Jakarta: IAARD Press; 2016.

Peraturan Menteri Pertanian Republik Indonesia nomor 14/Permentan/PK.350/5/2017 tentang Klasifikasi Obat Hewan. 25 hal.

FAO/WHO Food and Agriculture Organization of the United Nations/World Health Organization. 2002. Guidelines for evaluation of probiotics in food. http://www.who.int/foodsafety/fs_management/en/probotics_guidelines.pdf.

Seo JK, Kim SW, Kim MH, Upadhaya SD, Kam DK, Ha JK. Direct-fed microbials for ruminant animals. Asian Australasian Journal of Animal Science 2010; 23(12): 1657–1667.

McAllister TA, Okine EK, Mathison GW, Cheng KJ. Dietary, environmental and microbiological aspects of methane production in ruminants. Canadian Journal of Animal Science 1996; 76: 231–243.

Leng RA. Modification of rumen fermentation. Proceeding Nutritional Limit to Animal Production from Pastures. Commonwealth Agricultural Bureaux, St Lucia, Queensland Australia 1981: 427–453.

Soetanto H, Hendratno C, Widyastuti Y. Observasi pendahuluan komposisi mikroba rumen domba, kambing, kerbau dan sapi sebagai respons terhadap kualitas pakan yang berbeda. Prosiding Isotop dan Radiasi, Jakarta 1994: 43–52.

Henderson G, Cox F, Ganesh S, Jonker A, Young W, Janssen PH. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Scientific Reports 2015; 5: 14567.

Hungate RE. The rumen and its microbes. New York: Academic Press; 1966.

Krause DO, Denman SE, Mackie RI, Morrison M, Rae AL, Attwood GT, McSweeney CS. Opportunities to improve fiber degradation in the rumen: Microbiology, ecology, and genomics. FEMS Microbiologi Review 2003; 27(5): 663–93.

Widyastuti Y, Lee WK, Suzuki K, Mitsuoka T. Isolation and characterization of rice straw-degrading Clostridia from cattle rumen. Journal Veterinary and Medical Science 1992; 54(1): 185–188.

Widyastuti Y. Pengaruh adaptasi dan transfer terhadap aktivitas kecernaan Ruminococcus flavefaciens 17. Prosiding Seminar Bioteknologi, Bogor 1994: 313–319.

Widyastuti Y. Kecernaan dinding sel jerami padi oleh Ruminococcus flavefaciens 17, Fibrobacter succinogenes B12 dan Selenomonas ruminantium JW13. Prosiding Seminar Nasional Sains dan Teknologi, Bogor 1995: 120–125.

Budiansyah A, Resmi, Wiryawan KG, Soehartono MT, Widyastuti Y, Ramli N. Isolasi dan karakterisasi enzim karbohidrase cairan rumen sapi asal rumah potong hewan. Media Peternakan 2010; 33(1): 36–43.

Widyastuti Y, Newbold CJ, Stewart CS, Ørskov ER. Interaction between rumen anaerobic fungi and ciliate protozoa in the degradation of rice straw cell walls. Letter for Applied Microbiology 1995; 20(1): 61–64.

Takahashi J. Lactic acid bacteria and mitigation of GHG emission from ruminant livestock in lactic acid bacteria- R&D for Food, health and livestock purposes. London: Intech Open; 2013.

McSweeney C, Kang S, Gagen E, Davis C, Morrison M, Denman S. Recent developments in nucleic acid based techniques for use in rumen manipulation. Revista Brasileira de Zootecnia 2009; 38: 341–351.

Kumar S, Pitta DW. Revolution in rumen microbiology. Dalam: Rumen microbiology: From evolution to revolution. New Delhi: Springer; 2015. 357–379.

Ametaj BN, Zebeli Q, Saleem F, Psychogios N, Lewis MJ, Dunn SM, Xia J, Wishart DS. Metabolomics reveals unhealthy alterations in rumen metabolism with increased proportion of cereal grain in the diet of dairy cows. Metabolomics 2010; 6: 583–594.

Giraffa G, Chanishvili N, Widyastuti Y. Importance of lactobacilli in food and feed biotechnology. Research in Microbiology 2010; 161(6): 480–487.

Wang W, Wang HK. The effect of lactic acid bacteria in food and feed and their impact on food safety. International Journal of Food Engineering 2014; 10(2): 203–210. doi 10.1515/ijfe-2013-0042.

Widyastuti Y, Rohmatussolihat, Febrisiantosa A. The role of lactic acid bacteria in milk fermentation. Food and Nutrition Sciences 2014; 5: 435–442.

Widyastuti Y, Lisdiyanti P, Tisnadjaja D. Role of Lactobacillus helveticus on flavor formation in cheese: Amino acid metabolism. Annales Bogorienses 2014; 18(1): 1–11.

Rohmatussolihat, Sari MN, Lisdiyanti P, Widyastuti Y, Sukara E. Pemanfaatan milk clotting enzyme dari Lactobacillus casei D11 untuk pembuatan keju mozzarella. Jurnal Teknologi dan Industri Pangan 2015; 26(1): 63–71.

Widyastuti Y, Ratnakomala S, Ekawati F. Bakteri asam laktat pada buah-buahan tropis. Prosiding Seminar Mikrobiologi Indonesia, Bandar Lampung 1998: 447–458.

Vaughan EE, Heilig HGHJ, Ben-Amor K, de Vos WM. Diversity, vitality and activities of intestinal lactic acid bacteria and bifidobacteria assessed by molecular approaches. FEMS Microbiology Reviews 2005; 29: 477–490.

Widyastuti Y, Sofarianawati E. Karakteristik Enterococcus sp. yang diisolasi dari saluran pencernaan domba. Jurnal Mikrobiologi Indonesia 1999; 4(2): 50–53.

Widyastuti Y. Characteristics of lactic acid bacteria from the rumen of sheep. Annales Bogorienses 1995; 3(2): 25–32.

Stewart CS. Lactic acid bacteria in the rumen. Dalam: The lactic acid bacteria. London: Elsevier Applied Science; 1992. 49–68.

Widyastuti Y, Prayitno NR, Lisdiyanti P, Sukara E, Saono S. Culture collection in the Indonesian Institute of Sciences (LIPI). Proceeding of the Tenth International Congress for Culture Collections: Innovative Roles of Biological Resource Centers, Tokyo 2004: 503.

Saono S, Ganjar I, Harsojo, Hastiono S, Rahayu ES, Rahayu G, Soemara SI, Sudarmono P, Supardiyono EK, Widyastuti Y. Daftar biakan mikroorganisme Indonesia. Edisi pertama. Cibinong: Pusat Penelitian Bioteknologi; 1999.

Saono S, Prayitno NR, Oetari A, Harsojo, Hastiono S, Rahayu ES, Rahayu G, Soemara SI, Sudarmono P, Supardiyono EK, Udhiharto M, Wahyudi P, Wibowo MS, Widyastuti Y. Daftar biakan mikroorganisme Indonesia. Edisi kedua. Cibinong: Pusat Penelitian Bioteknologi; 2000.

Yamada Y, Katsura K, Kawasaki H, Widyastuti Y, Saono S, Seki T, Uchimura T, Komagata K. Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the -Proteobacteria. International Journal of Systematic and Evolutionary Microbiology 2000; 50: 823-829.

Lisdiyanti, P, Kawasaki H, Widyastuti Y, Saono S, Seki T, Yamada Y, Uchimura T, Komagata K. Kozakia baliensis gen. nov., sp. nov., a new acetic acid bacterium in the -Proteobacteria. International Journal of Systematic and Evolutionary Microbiology 2002; 52: 813–818.

Yamada Y, Hosono R, Lisdyanti P, Widyastuti Y, Saono S, Uchimura T, Komagata K. Identification of acetic acid bacteria isolated from Indonesian sources, especially of isolates classified in the genus Gluconobacter. Journal of General and Applied Microbiology 1999; 45: 23–28.

Ando K, Widyastuti Y, Pawiroharsono S, Soebandrio A. Biodiversity Research under CBD: A case study of Indonesia-NITE Joint Research Project. Proceeding of the Tenth International Congress for Culture Collection. Innovative Roles of Biological Resource Centers-Tsukuba, Japan 2004: 173–175.

Otoguro M, Ratnakomala S, Lestari Y, Hastuti RD, Triana E, Widyastuti Y, Ando K. Streptomyces baliensis sp. nov., isolated from Balinese soil. International Journal of Systematic and Evolutionary Microbiology 2009; 59: 2158–2161.

Yamamura H, Lisdiyanti P, Ridwan R, Ratnakomala S, Saraswati R, Lestari Y, Triana E, Kartina G, Widyastuti Y, Ando K. Dietzia timorensis sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology 2010; 60: 451–454.

Lisdiyanti P, Otoguro M, Ratnakomala S, Lestari Y, Hastuti RD, Triana E, Ando K, Widyastuti Y. Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov., Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter. International Journal of Systematic and Evolutionary Microbiology 2010; 60: 2331–2335.

Otoguro M, Yamamura H, Tamura T, Irzaldi R, Ratnakomala S, Ridwan R, Kartina G, Triana E, Nurkanto A, Lestari Y, Lisdiyanti P, Widyastuti Y, Ando K. Actinophytocola timorensis sp. nov. and Actinophytocola corallina sp. nov., isolated from soil in Indonesia. International Journal of Systematic and Evolutionary Microbiology 2011; 61: 834–838.

Nakashima C, Oetari A, Kanti A, Saraswati R, Widyastuti Y, Ando K. New species and newly record species of Cercospora and allied genera from Indonesia. Mycosphere 2010; 1(4): 315–323.

Sjamsuridzal W, Oetari A, Nakashima C, Kanti A, Saraswati R, Widyastuti Y, Ando K. New species of the genus Metschnikowia isolated from flowers in Indonesia, Metschnikowia cibodasensis sp. nov. Journal of Microbiology and Biotechnology 2013; 23(7): 905–912.

Widyastuti Y, Lisdiyanti P, Ratnakomala S, Kartina G, Ridwan R, Rohmatussolihat, Prayitno NR, Triana E, Widhyastuti N, Saraswati R, Hastuti RD, Lestari Y, Otoguro M, Miyadoh S, Yamamura H, Tamura T, Ando K. Genus diversity of actinomycetes in Cibinong Science Center, West Java, Indonesia. Microbiology Indonesia 2012; 6(4): 165–172.

Lisdiyanti P, Tamura T, Ratnakomala S, Ridwan R, Kartina G, Lestari Y, Katsuhiko A, Widyastuti Y. Diversity of actinomycetes from soil samples collected from Lombok Island, Indonesia. Annales Bogorienses 2015, 16(1): 35–40.

Ratnakomala S, Lisdiyanti P, Widyastuti Y. Collection of Indonesian actinomycetes and its uses. Dalam: Exploring Indonesian microbial genetic resources for industrial application. Jakarta: LIPI Press; 2016. 177–203.

Sukara E, Lisdiyanti P. Exploring mirobial genetic resources for industrial application. Jakarta: LIPI Press; 2016.

Widyastuti, Y. Lactic acid bacteria from fermented food and feed. Dalam: Exploring mirobial genetic resources for industrial application. Jakarta: LIPI Press; 2016. 75–89.

Astuti WD, Widyastuti Y, Wina E, Suharti S, Ridwan R, Wiryawan KG. Survival of Lactobacillus plantarum U40 on the in vitro rumen fermentation quantified with real-time PCR. Journal of the Indonesian Tropical Animal Agriculture 2018; 43(2): 184–192.

Sofyan A, Yusiati LM, Widyastuti Y, Utomo R. Microbiological characteristic and fermentability of King grass (Pennisetum hybrid) silage treated by lactic acid bacteria-yeast inoculants consortium combined with rice bran addition. Journal of the Indonesian Tropical Animal Agriculture 2011; 36(4): 265–272.

Sofyan A, Widyastuti Y, Utomo R, Yusiati LM. Improving physicochemical characteristic and palatability of King grass (Pennisetum hybrid) silage by inoculation of Lactobacillus plantarum-Saccharomyces cerevisiae consortia and addition of rice bran. Buletin Peternakan 2017; 41(1): 61–71.

Sofyan A, Widyastuti Y, Utomo R, Yusiati LM. Sediaan inokulum silase. Paten Indonesia No. IDP000049529. 9 Februari 2018.

Li M, Zi X, Zhou H, Lv R, Tang J, Cai Y. Silage fermentation and ruminal degradation of cassava foliage prepared with microbial additive. AMB Express 2019; 9: 180–185.

Kim JG, Ham JS, Li YW, Park HS, Huh CS, Park BC. Development of a new lactic acid bacterial inoculant for fresh rice straw silage. Asian-Australasian Journal of Animal Science 2017; 30(7): 950–956.

Ridwan. R, Widyastuti Y. Membuat silase: Upaya mengawetkan dan mempertahankan nilai nutrisi hijauan pakan ternak. Warta Biotek-LIPI 2001; 15(1): 9–14.

Ridwan R, Ratnakomala S, Kartina G, Widyastuti Y. Pengaruh penambahan dedak padi dan Lactobacillus plantarum 1 Bl-2 dalam pembuatan silase rumput gajah (Pennisetum purpureum). Media Peternakan 2005; 28(3): 1176–123.

Ratnakomala S, Ridwan R, Kartina G, Widyastuti Y. Pengaruh inokulum Lactobacillus plantarum 1A-2 dan 1 BL-2 terhadap kualitas silase rumput gajah (Pennisetum purpureum). Biodiversitas 2006; 7(2): 131–134.

Hapsari SS, Suryahadi, Sukria HA. Improvement on the nutritive quality of Napier grass silage through inoculation of Lactobacillus plantarum and formic acid. Media Peternakan 2016; 39(2): 125–133.

Astuti WD, Widyastuti Y, Ridwan R. Preservation of elephant grass and vegetable waste using Lactobacillus plantarum 1A-2 as silage inoculant. Proceedings International Conference on Biotechnology, Universitas Padjadjaran, Bandung 2013: 57–65.

Astuti WD, Widyastuti Y, Ridwan R, Yetti E. Quality of vegetable waste silages treated with various carbohydrate sources. Media Peternakan 2013; 36(2): 120–125.

Yetti E, Ridwan R, Susilaningsih D, Yopi, Rahmani N, Astuti WD, Widyastuti Y. Quality of silase based on local feed resources in Lombok and its influence to cattle productivity in Banyumulek, Nusa Tenggara Barat (NTB), Indonesia. Proceedings International Conference on Biotechnology, Pusat Penelitian Bioteknologi, Bogor 2013: 49–56.

Sidiq F, Widyastuti Y, Putri FJ, Ridwan R. Pengaruh taraf inklusi legum Acacia villosa terhadap kualitas silase gabungan rumput-legum yang diberi aditif berupa Lactobacillus plantarum 1A-2 dan dedak padi. Prosiding Seminar Peternakan, Pusat Penelitian Bioteknologi, Bogor 2013: 450–468.

Putri FJ, Sidiq F, Ridwan R, Widyastuti Y. Kecernaan bahan kering dan bahan organik silase campuran Pennisetum purpureum dan Acacia villosa secara in vitro. Prosiding Seminar Peternakan, Pusat Penelitian Bioteknologi, Bogor 2013: 400–408.

Ridwan R, Rusmana I, Widyastuti Y, Wiryawan KG, Prasetya B, Sakamoto M, Ohkuma M. Fermentation characteristics and microbial diversity of tropical grass-legumes silages. Asian Australasian Journal of Animal Science 2015; 28(4): 511–518.

Ridwan R, Rusmana I, Widyastuti Y, Wiryawan KG, Prasetya B, M. Sakamoto, and M. Ohkuma. Methane mitigation and microbial diversity of silage diets containing Calliandra calothyrsus in a rumen in vitro fermentation system. Media Peternakan 2014; 37(2):121–128.

Ridwan R, Rusmana I, Widyastuti Y, Wiryawan KG, Prasetya B, Sakamoto M, Ohkuma M. Bacteria and methanogen community in the rumen fed different levels of grass-legume silages. Biodiversitas 2019; 20(4): 1055–1062.

Komalasari K, Astuti DA, Widyastuti Y, Astuti WD, Ridwan R. Rumen fermentation and milk quality of dairy cows fed complete feed silages. Media Peternakan 2014; 37(1): 38–42.

Widyastuti Y, Ratnakomala S, Rachmat J, Sofarianawati E. Studies on the characteristics growth of Lactobacillus sp. a candidate for probiotics. Annales Bogorienses 2001; 6(2): 103–108.

Widyastuti Y, Ratnakomala S, Sofarianawati E, Rachmat J. Kultur campuran bakteri asam laktat sebagai probiotik untuk ruminansia. Prosiding Seminar Nasional Peternakan dan Veteriner, Bogor 2000: 336–348.

Ridwan R, Bungsu WA, Astuti, WD, Rohmatussolihat, Sari NF, Fidriyanto R, Jayanegara A, Wijayanti I, Widyastuti Y. The use of lactic acid bacteria as ruminant probiotic candidates based on in vitro fermentation characteristics. Buletin Peternakan 2018; 42(1): 31–36.

Astuti WD, Wiryawan KG, Wina E, Widyastuti Y, Suharti S, Ridwan R. Effects of selected Lactobacillus plantarum as probiotic on in vitro ruminal fermentation and microbial population. Pakistan Journal of Nutrition 2018; 17(3): 131–139.

Sari NF, Ridwan R, Rohmatussolihat, Fidriyanto R, Astuti WD, Widyastuti Y. The effect of probiotics on high fiber diet in rumen fermentation characteristics. IOP Conference Series Earth Environmetal Science 2019: 251 012057

Ratnakomala S, Widyastuti Y. Storage of Probiotic Leuconostoc citreum TSD-10 by freezing and freeze-drying. Annales Bogorienses 2000; 7(1): 43–48.

Ridwan R, Rohmatussolihat, Widyastuti Y, Ratnakomala S, Astuti WD, Fidryanto R, Sari NF. Komposisi medium pertumbuhan bakteri asam laktat untuk probiotik ruminansia. Paten Indonesia No. IDP000066683. 21 Januari 2020.

Widyastuti Y, Sari NF, Fidriyanto R, Astuti WD, Rohmatussolihat, Ridwan R. Teknologi pengolahan pakan sapi potong di Technopark Banyumulek, Nusa Tenggara Barat. Jakarta: LIPI Press; 2020.

Fidriyanto R, Ridwan R, Astuti WD, Rohmatussolihat, Sari NF, Widyastuti Y. Komposisi medium aktivasi bakteri probiotik dan proses pembuatannya. Paten Indonesia No. IDP000077873. 9 Juli 2021.

Rohmatussolihat, Ridwan R, Widyastuti Y, Fidriyanto R, Astuti WD, Sari NF. Formulasi probiotik serbuk untuk ternak ruminanisa. Paten Indonesia No. P00201707656. 31 Oktober 2017.

Widyastuti Y, Rohmatussolihat, Fidriyanto R, Ridwan R, Sari NF, Astuti WD. Formulasi inokulum probiotik serbuk dengan metode kering beku untuk ternak ruminansia. Paten Indonesia No. P00201809255. 15 November 2018.

Van Soest PJ. Development of a comprehensive system of feed analyses and its application to forage. Journal of Animal Science 1967; 26: 119–128.

Abe A, Horii S, Kameoka K. Application of enzymatic analysis with glucoamylase, pronase and cellulase to various feeds for cattle. Journal of Animal Science 1979; 48(6): 1483–1490.

Widyastuti Y, Terada F, Kajikawa H, Abe A. Digestion of rice straw cell wall constituents in various rumen conditions. Japan Agriculture Research Quarterly 1987; 21(1): 59–64.

Widyastuti Y, Abe A. Effect of the silica content on digestibility of rice straw. Japan Agriculture Research Quarterly 1989; 23(1): 53–58.

Sari NF, Ridwan R, Rohmatussolihat, Fidriyanto R, Astuti WD, Widyastuti Y. Characteristic of different level of fermented concentrate in the rumen metabolism based on in vitro. Journal of the Indonesian Tropical Animal Agriculture 2018; 43(3): 296–305.

Imsya A, Laconi EB, Wiryawan KG, Widyastuti Y. In vitro digestibility of ration containing different level of palm oil frond fermented with Phanerochaete chrysosporium. Media Peternakan 2013; 36(2): 131–136.

Astuti WD, Widyastuti Y, Fidriyanto R, Ridwan R, Rohmatussolihat, Sari NF, Firsoni, Sugoro I. In vitro gas production and digestibility of oil palm frond silage mixed with different levels of elephant grass. Institute of Physics Conference. Series: Earth and Environmental Science 2020: 439: 012022.

Ridwan R, Widyastuti Y, Budiarti S, Dinoto A. Effectivity of silage and probiotic on the rumen metabolism of ongole cattle in vivo experiment. Proceeding Seminar Feed Safety for Healthy Food, Bandung 2011: 368–375.

Sari NF, Ridwan R, Widyastuti Y. The quality of corn silage product from Technopark of Banyumulek Lombok, West Nusa Tenggara. Buletin Peternakan 2017; 41(2): 156–162.

Downloads

Download PDF

Published

August 27, 2021

Series

Orasi Pengukuhan Profesor Riset

Categories

Copyright (c) 2021 Lembaga Ilmu Pengetahuan Indonesia

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Details about this monograph

ISBN-13 (15)

978-602-496-247-0